Insertion of short hepatitis virus A amino acid sequences into poliovirus antigenic determinants results in viable progeny

In an infectious poliovirus cDNA construct, the determinant encoding antigenic epitope N-Agl (in a loop located between two β-strands in polypeptide VP1) was altered by site-directed mutagenesis, to be partially similar with the determinants for presumptive epitopes in polypeptides VP1 or VP3 of hep...

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Bibliographic Details
Published in:FEBS Letters 1989-11, Vol.257 (2), p.354-356
Main Authors: Sverdlov, E.D., Tsarev, S.A., Markova, S.V., Rostapshov, V.M., Azhikina, T.L., Chernov, I.P., Gorbalenya, A.E., Kolesnikova, M.S., Romanova, L.I., Teterina, N.L., Tolskaya, E.A., Agol, V.I.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
cDNA, infectious
Cells, Cultured
Chimeric virus
DNA - genetics
Epitopes
Fundamental and applied biological sciences. Psychology
Genetics
Haplorhini
Hepatits A virus
Hepatovirus - genetics
Hepatovirus - immunology
Microbiology
Molecular Sequence Data
Plasmids
Poliovirus
Poliovirus - genetics
Poliovirus - growth & development
Poliovirus - immunology
Poliovirus Vaccine, Oral - genetics
Restriction Mapping
Vaccines - genetics
Vaccines, Synthetic - genetics
Viral Vaccines - immunology
Virology
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Summary:In an infectious poliovirus cDNA construct, the determinant encoding antigenic epitope N-Agl (in a loop located between two β-strands in polypeptide VP1) was altered by site-directed mutagenesis, to be partially similar with the determinants for presumptive epitopes in polypeptides VP1 or VP3 of hepatitis A virus (HAV). The modified constructs proved to be infectious. However, another construct, in which the same locus encoded a ‘nonsense’ and a relatively hydrophobic amino acid sequence, exhibited no infectivity. These data showed the feasibility of the insertion of foreign sequences in a specific antigenically active locus of the poliovirus icosahedron, and suggest some limitations with respect to the sequences to be ‘transplanted’.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(89)81570-4