Ornamental fish as a source of plasmid-mediated quinolone resistance genes and antibiotic resistance plasmids

Growing ornamental fish industry is associated with public health concerns including extensive antibiotic use accompanied by increasing antibiotic resistance. The aim of this study was to analyze Aeromonas isolates from imported tropical ornamental fish and coldwater koi carps bred in the Czech Repu...

Full description

Saved in:
Bibliographic Details
Published in:Veterinary microbiology 2014-07, Vol.171 (3-4), p.413-421
Main Authors: Dobiasova, Hana, Kutilova, Iva, Piackova, Veronika, Vesely, Tomas, Cizek, Alois, Dolejska, Monika
Format: Article
Language:English
Subjects:
Aeromonas
Aeromonas - genetics
Animals
Anti-Bacterial Agents - pharmacology
Aquaculture
Base Sequence
Blotting, Southern - veterinary
Carps - microbiology
Ciprofloxacin - pharmacology
Czech Republic
DNA Primers - genetics
Drug Resistance, Bacterial - genetics
Fish Diseases - microbiology
Freshwater
Gram-Negative Bacterial Infections - genetics
Gram-Negative Bacterial Infections - veterinary
Humans
Microbial Sensitivity Tests - veterinary
Molecular Sequence Data
Ornamental fish
Plasmids
Plasmids - genetics
Polymerase Chain Reaction - veterinary
Quinolone resistance
Sequence Analysis, DNA - veterinary
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Growing ornamental fish industry is associated with public health concerns including extensive antibiotic use accompanied by increasing antibiotic resistance. The aim of this study was to analyze Aeromonas isolates from imported tropical ornamental fish and coldwater koi carps bred in the Czech Republic to assess the potential risk of ornamental fish as a source of plasmid-mediated quinolone resistance genes (PMQR) and antibiotic resistance plasmids. A collection of Aeromonas spp. with reduced susceptibility to ciprofloxacin (MIC≥0.05mg/L) was selected for the detection of PMQR genes. Isolates harbouring PMQR genes were further analyzed for the additional antibiotic resistance, integron content, clonality, biofilm production and transferability of PMQR genes by conjugation and transformation. Comparative analysis of plasmids carrying PMQR genes was performed. Fifteen (19%, n=80) isolates from koi carps and 18 (24%, n=76) isolates from imported ornamental fish were positive for qnrS2, aac(6′)-Ib-cr or qnrB17 genes. PMQR-positive isolates from imported ornamental fish showed higher MIC levels to quinolones, multiresistance and diverse content of antibiotic resistance genes and integrons compared to the isolates from the carps. Related IncU plasmids harbouring qnrS2 and aac(6′)-Ib-cr genes were found in Aeromonas spp. from imported ornamental fish and koi carps from various geographical areas. Ornamental fish may represent a potential source of multiresistant bacteria and mobile genetic elements for the environment and for humans.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2014.02.011