Enzymatic saccharification of pretreated rice straw by cellulase produced from Bacillus carboniphilus CAS 3 utilizing lignocellulosic wastes through statistical optimization

A marine bacterium, Bacillus carboniphilus CAS 3 was subjected to optimization for cellulase production utilizing cellulosic waste through response surface methodology. Plackett – Burman and Central composite design was employed and the optimal medium constituents for maximum cellulase production (4...

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Bibliographic Details
Published in:Biomass & bioenergy 2014-09, Vol.68, p.151-160
Main Authors: Annamalai, Neelamegam, Rajeswari, Mayavan Veeramuthu, Balasubramanian, Thangavel
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Bacillus
Bacillus carboniphilus
Biological and medical sciences
Biotechnology
Cellulase
Cellulosic wastes
Enzyme engineering
Food industries
Fundamental and applied biological sciences. Psychology
General agronomy. Plant production
Methods. Procedures. Technologies
Oryza sativa
Production of selected enzymes
Response surface methodology
Saccharification
Use and upgrading of agricultural and food by-products. Biotechnology
Use of agricultural and forest wastes. Biomass use, bioconversion
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Summary:A marine bacterium, Bacillus carboniphilus CAS 3 was subjected to optimization for cellulase production utilizing cellulosic waste through response surface methodology. Plackett – Burman and Central composite design was employed and the optimal medium constituents for maximum cellulase production (4040.45 U/mL) were determined as rice bran, yeast extract, MgSO4·7H2O and KH2PO4 at 6.27, 2.52, 0.57 and 0.39 g/L, respectively. The cellulase produced was purified to the specific activity of 434.94 U/mg and 11.46% of recovery with the molecular weight of 56 kDa. The optimum temperature, pH and NaCl for enzyme activity was determined as 50 °C, 9 and 30% and more than 70% of its original activity was retained even at 80 °C, 12 and 35% respectively. Further, enzymatic saccharification of pretreated rice straw yielded about 15.56 g/L of reducing sugar at 96 h, suggesting that the purified cellulase could be useful for production of reducing sugars from cellulosic biomass into ethanol. •Bacillus carboniphilus CAS 3 produced cellulase from cellulosic waste through statistical optimization.•A significantly higher cellulase production (4168 U/mL) was achieved under optimized conditions.•Higher stability towards temperature, pH and NaCl could be useful for industrial application.•Enzymatic saccharification of pre-treated rice straw yielded 15.56 g/L reducing sugars.•Significant increase in sugar yield from cellulosic biomass could be useful in ethanol production.
ISSN:0961-9534
1873-2909
DOI:10.1016/j.biombioe.2014.06.018