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Signal transducer and activator of transcription 3 (STAT3) mutations underlying autosomal dominant hyper-IgE syndrome impair human CD8 super(+) T-cell memory formation and function
Background: The capacity of CD8 super(+) T cells to control infections and mediate antitumor immunity requires the development and survival of effector and memory cells. IL-21 has emerged as a potent inducer of CD8 super(+) T-cell effector function and memory development in mouse models of infectiou...
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Published in: | Journal of allergy and clinical immunology 2013-08, Vol.132 (2), p.400-411.e9 |
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Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Online Access: | Get full text |
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Summary: | Background: The capacity of CD8 super(+) T cells to control infections and mediate antitumor immunity requires the development and survival of effector and memory cells. IL-21 has emerged as a potent inducer of CD8 super(+) T-cell effector function and memory development in mouse models of infectious disease. However, the role of IL-21 and associated signaling pathways in protective CD8 super(+) T-cell immunity in human subjects is unknown. Objective: We sought to determine which signaling pathways mediate the effects of IL-21 on human CD8 super(+) T cells and whether defects in these pathways contribute to disease pathogenesis in patients with primary immunodeficiencies caused by mutations in components of the IL-21 signaling cascade. Methods: Human primary immunodeficiencies resulting from monogenic mutations provide a unique opportunity to assess the requirement for particular molecules in regulating human lymphocyte function. Lymphocytes from patients with loss-of-function mutations in signal transducer and activator of transcription 1 (STAT1), STAT3, or IL-21 receptor (IL21R) were used to assess the respective roles of these genes in human CD8 super(+) T-cell differentiation in vivo and in vitro. Results: Mutations in STAT3 and IL21R, but not STAT1, led to a decrease in multiple memory CD8 super(+) T-cell subsets in vivo, indicating that STAT3 signaling, possibly downstream of IL-21R, regulates the memory cell pool. Furthermore, STAT3 was important for inducing the lytic machinery in IL-21-stimulated naive CD8 super(+) T cells. However, this defect was overcome by T-cell receptor engagement. Conclusion: The IL-21R/STAT3 pathway is required for many aspects of human CD8 super(+) T-cell behavior but in some cases can be compensated by other signals. This helps explain the relatively mild susceptibility to viral disease observed in STAT3- and IL-21R-deficient subjects. |
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ISSN: | 0091-6749 |
DOI: | 10.1016/j.jaci.2013.05.029 |