Molecular and functional characterization of wheat near‐isogenic line ‘i:S29Ra’ having intensive anthocyanin pigmentation of the coleoptile, culm, leaves and auricles

Near‐isogenic line of the common spring wheat cultivar ‘Saratovskaya 29’, designated ‘i:S29Ra’, has been earlier developed during eight backcrosses and selection for the red auricle colour inherited from donor ‘Ulyanovka’. In this study, the line ‘i:S29Ra’ was characterized using 274 molecular and a...

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Bibliographic Details
Published in:Plant breeding 2014-08, Vol.133 (4), p.454-458
Main Authors: Khlestkina, Elena K, Gordeeva, Elena I, Arbuzova, Valentina S, Pillen, K
Format: Article
Language:English
Subjects:
absorbance
anthocyanin pigmentation
anthocyanins
backcrossing
Biosynthesis
coleoptiles
color
cultivars
Genes
genotyping
inheritance (genetics)
leaves
loci
microsatellite
microsatellite repeats
pigmentation
SNP
spring wheat
structural genes
transcription
Triticum aestivum
wheat near-isogenic lines
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Summary:Near‐isogenic line of the common spring wheat cultivar ‘Saratovskaya 29’, designated ‘i:S29Ra’, has been earlier developed during eight backcrosses and selection for the red auricle colour inherited from donor ‘Ulyanovka’. In this study, the line ‘i:S29Ra’ was characterized using 274 molecular and a number of morphological markers. It was found that the donor's genome material in ‘i:S29Ra’ is presented by a fragment on chromosome 7D flanked by the microsatellite loci Xgwm0044 and Xgwm1481 and carrying cluster of the genes Rc‐D1 (red coleoptile), Pc‐D1 (purple culm), Pls‐D1 (purple leaf sheaths), Plb‐D1 (purple leaf blades) and Ra‐D1 (red auricles), conferring intensive anthocyanin pigmentation of the coleoptile, culm, leaf sheaths, leaf blades and auricles, respectively. The Ra‐D1 is a novel locus described in wheat. The relative anthocyanin contents (OD₅₃₀) in different parts of wheat plant and expression level of the anthocyanin biosynthesis (AB) structural genes in auricles were compared between ‘i:S29Ra’ and ‘S29’. In auricles of ‘i:S29Ra’, the OD₅₃₀ (optical density) value was twice higher and transcription of some AB structural genes was increased in comparison with ‘S29’, suggesting regulatory role of the Ra gene in anthocyanin biosynthesis in wheat.
ISSN:0179-9541
1439-0523
DOI:10.1111/pbr.12180