A partial deletion in non-structural protein 3A can attenuate foot-and-mouth disease virus in cattle

Abstract The role of non-structural protein 3A of foot-and-mouth disease virus (FMDV) on the virulence in cattle has received significant attention. Particularly, a characteristic 10–20 amino acid deletion has been implicated as responsible for virus attenuation in cattle: a 10 amino acid deletion i...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2013-11, Vol.446 (1), p.260-267
Main Authors: Pacheco, Juan M, Gladue, Douglas P, Holinka, Lauren G, Arzt, Jonathan, Bishop, Elizabeth, Smoliga, George, Pauszek, Steve J, Bracht, Alexa J, O'Donnell, Vivian, Fernandez-Sainz, Ignacio, Fletcher, Paige, Piccone, Maria E, Rodriguez, Luis L, Borca, Manuel V
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cattle
Cattle Diseases - pathology
Cattle Diseases - virology
Foot-and-Mouth Disease - pathology
Foot-and-Mouth Disease - virology
Foot-and-mouth disease virus
Foot-and-Mouth Disease Virus - genetics
Foot-and-Mouth Disease Virus - pathogenicity
Infectious Disease
Molecular Sequence Data
Nasal Mucosa - virology
Sequence Alignment
Sequence Deletion
Serum - virology
Viral Load
Viral Nonstructural Proteins - genetics
Viral Nonstructural Proteins - metabolism
Viral Plaque Assay
Virulence
Virulence Factors - genetics
Virulence Factors - metabolism
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Summary:Abstract The role of non-structural protein 3A of foot-and-mouth disease virus (FMDV) on the virulence in cattle has received significant attention. Particularly, a characteristic 10–20 amino acid deletion has been implicated as responsible for virus attenuation in cattle: a 10 amino acid deletion in the naturally occurring, porcinophilic FMDV O1 Taiwanese strain, and an approximately 20 amino acid deletion found in egg-adapted derivatives of FMDV serotypes O1 and C3. Previous reports using chimeric viruses linked the presence of these deletions to an attenuated phenotype in cattle although results were not conclusive. We report here the construction of a FMDV O1Campos variant differing exclusively from the highly virulent parental virus in a 20 amino acid deletion between 3A residues 87–106, and its characterization in vitro and in vivo . We describe a direct link between a deletion in the FMDV 3A protein and disease attenuation in cattle.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2013.08.003