Assay of free-radical toxicity and antioxidant effect on the Hep 3B cell line : a test survey using lindane

The content of reduced glutathione and of glutathione disulfide as well as the activities of glutathione reductase, glutathione peroxidase, glutathione S-transferases, catalase and superoxide dismutases were determined in human hepatoma Hep 3B cells in relation to free-radical toxicity in order to a...

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Bibliographic Details
Published in:Cell biology and toxicology 1996-02, Vol.12 (1), p.19-28
Main Authors: DESCAMPIAUX, B, LEROUX, J. M, PEUCELLE, C, ERB, F
Format: Article
Language:English
Subjects:
Antioxidants - toxicity
Biological and medical sciences
Carcinoma, Hepatocellular - pathology
Free Radicals - toxicity
Humans
Lindane - toxicity
Liver - pathology
Medical sciences
Oxidation-Reduction
Pesticides, fertilizers and other agrochemicals toxicology
Superoxide Dismutase - analysis
Superoxide Dismutase - drug effects
Toxicology
Tumor Cells, Cultured
Vitamin E - toxicity
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Summary:The content of reduced glutathione and of glutathione disulfide as well as the activities of glutathione reductase, glutathione peroxidase, glutathione S-transferases, catalase and superoxide dismutases were determined in human hepatoma Hep 3B cells in relation to free-radical toxicity in order to appreciate the defense capacities of these cells compared to data on normal hepatocytes. When Hep 3B cells were exposed to lindane, a known inducer of free-radical production, superoxide dismutase activity appeared as the best-adapted cellular parameter for early detection of the resulting free-radical toxicity.
ISSN:0742-2091
1573-6822
DOI:10.1007/BF00143391