Complete nucleotide sequence and genome organization of an endornavirus from bottle gourd (Lagenaria siceraria) in California, U.S.A

The full-length nucleotide sequence and genome organization of an Endornavirus isolated from ornamental hard shell bottle gourd plants (Lagenaria siceraria (Molina) Standl.) in California (CA), USA tentatively named L. siceraria endornavirus-California (LsEV-CA) was determined. The LsEV-CA genome wa...

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Bibliographic Details
Published in:Virus genes 2014-08, Vol.49 (1), p.163-168
Main Authors: Kwon, Sun-Jung, Tan, Shih-hua, Vidalakis, Georgios
Format: Article
Language:English
Subjects:
3' Untranslated Regions
5' Untranslated Regions
Base Composition
Biomedical and Life Sciences
Biomedicine
California
Cucurbitaceae - virology
Endornavirus
Gene Order
genome
Genome, Viral
Lagenaria siceraria
Medical Microbiology
Molecular Sequence Data
Molecular Weight
Open Reading Frames
Plant Sciences
Plant Viruses - genetics
Plant Viruses - isolation & purification
Polyproteins - chemistry
Polyproteins - genetics
RNA Viruses - genetics
RNA Viruses - isolation & purification
RNA, Viral - genetics
RNA-directed RNA polymerase
seedlings
Sequence Analysis, DNA
Virology
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Summary:The full-length nucleotide sequence and genome organization of an Endornavirus isolated from ornamental hard shell bottle gourd plants (Lagenaria siceraria (Molina) Standl.) in California (CA), USA tentatively named L. siceraria endornavirus-California (LsEV-CA) was determined. The LsEV-CA genome was 15088 bp in length, with a G + C content of 36.55 %. The lengths of the 5′ and 3′ untranslated regions were 111 and 52 bp, respectively. The genome of LsEV-CA contained one large ORF encoding a 576 kDa polyprotein. The predicted protein contains two glycosyltransferase motifs, as well as RNA-dependent RNA polymerase and helicase domains. LsEV-CA was detected in healthy-looking field-grown gourd plants, as well as plants expressing yellows symptoms. It was also detected in non-symptomatic greenhouse-grown gourd seedlings grown from seed obtained from the same field sites. These preliminary data indicate that LsEV-CA is likely not associated with the gourd-yellows syndrome observed in the field.
ISSN:0920-8569
1572-994X
DOI:10.1007/s11262-014-1064-x