Studies on the structure of the bioregulator purified from the rat brain

From the brain tissue of Wistar rats, we purified a bioregulator, which is active at ultralow doses. Using reversed-phase HPLC, we prepared a homogenous polypeptide with a molecular weight of 4749 ± 2 Da, which is responsible for the biological activity of the bioregulator. Using the CD spectroscopy...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2014-07, Vol.50 (4), p.398-403
Main Authors: Il’ina, A. P, Molyavka, A. A, Yamskova, V. P, Buryak, A. K, Yamskov, I. A
Format: Article
Language:English
Subjects:
bioactive properties
Biochemistry
Biomedical and Life Sciences
Brain
Life Sciences
Medical Microbiology
Microbiology
molecular weight
Polypeptides
Proteins
proteomics
rats
reversed-phase high performance liquid chromatography
Rodents
spectroscopy
Tissues
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Summary:From the brain tissue of Wistar rats, we purified a bioregulator, which is active at ultralow doses. Using reversed-phase HPLC, we prepared a homogenous polypeptide with a molecular weight of 4749 ± 2 Da, which is responsible for the biological activity of the bioregulator. Using the CD spectroscopy method, we calculated the percentage of canonical elements of the secondary polypeptide structure in a solution. Using the methods of proteomics, we revealed that the structure of the investigated polypeptide was similar to the N-terminal sequence of a fragment of guanine-nucleotide binding G₀-protein subunit α-1.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683814030077