A comparison of osteocyte bioactivity in fine particulate bone powder grafts vs larger bone grafts in a rat bone repair model

The osteogenic potential for bone grafts is based on numbers and activities of cells that survive transplantation. In this study, we compared the bioactivity of osteocytes in 300–500μm fine particulate bone powder grafts to 2mm larger bone grafts in a rat radial defect model. Expression levels of bo...

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Published in:Acta histochemica 2014-07, Vol.116 (6), p.1015-1021
Main Authors: Sun, Yuan-Xin, Sun, Cheng-Li, Tian, Ye, Xu, Wen-Xiao, Zhou, Chang-Long, Xi, Chun-Yang, Yan, Jing-Long, Wang, Xin-Tao
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - metabolism
Animals
Bioactivity
Bone and Bones - physiology
Bone grafts
Bone Morphogenetic Protein 2 - genetics
Bone Morphogenetic Protein 2 - metabolism
Bone powder
Bone Regeneration
Bone Transplantation
Collagen Type I - metabolism
Gene Expression
Male
Osteocytes
Osteocytes - physiology
Powders
Rat radial defect model
Rats, Inbred Strains
Transforming Growth Factor beta1 - genetics
Transforming Growth Factor beta1 - metabolism
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Summary:The osteogenic potential for bone grafts is based on numbers and activities of cells that survive transplantation. In this study, we compared the bioactivity of osteocytes in 300–500μm fine particulate bone powder grafts to 2mm larger bone grafts in a rat radial defect model. Expression levels of bone morphogenetic protein-2 (BMP-2), transforming growth factor-beta 1 (TGF-β1), alkaline phosphatase (ALP), and collagen I were semi-quantified by both immunohistochemistry and RT-PCR at days 1 and 4, as well as weeks 1, 2, 4, 6 and 10 post-transplantation. Within two weeks post-transplantation, more cells stained positively for BMP-2, TGF-β1, ALP, and collagen I within the bone grafts and in the surrounding tissues in the group transplanted with the fine particulate bone powder grafts than in those with larger bone grafts (P
ISSN:0065-1281
1618-0372
DOI:10.1016/j.acthis.2014.04.004