MicroRNA expression analysis and Multiplex ligation‐dependent probe amplification in metastatic and non‐metastatic uveal melanoma

Purpose To determine the association of microRNA expression and chromosomal changes with metastasis and survival in uveal melanoma (UM). Methods Thirty‐six patients with UM were selected based on the metastatic status, and clinicopathological data were collected. Multiplex ligation‐dependent probe a...

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Published in:Acta ophthalmologica (Oxford, England) England), 2014-09, Vol.92 (6), p.541-549
Main Authors: Larsen, Ann‐Cathrine, Holst, Line, Kaczkowski, Bogumil, Andersen, Morten T., Manfé, Valentina, Siersma, Volkert D., Kolko, Miriam, Kiilgaard, Jens F., Winther, Ole, Prause, Jan U., Gniadecki, Robert, Heegaard, Steffen
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
chromosomal aberrations
Chromosome Aberrations
Chromosomes, Human, Pair 3 - genetics
Chromosomes, Human, Pair 8 - genetics
expression
Female
Gene Expression Profiling
genetic
Humans
Lymphatic Metastasis
Male
Melanoma - genetics
Melanoma - mortality
Melanoma - secondary
metastasis
microRNA
MicroRNAs - genetics
Middle Aged
Multiplex ligation‐dependent probe amplification
Multiplex Polymerase Chain Reaction
Neoplasm Proteins - genetics
Ophthalmology
prognostic factors
Proportional Hazards Models
survival
Survival Rate
uveal melanoma
Uveal Neoplasms - genetics
Uveal Neoplasms - mortality
Uveal Neoplasms - pathology
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Summary:Purpose To determine the association of microRNA expression and chromosomal changes with metastasis and survival in uveal melanoma (UM). Methods Thirty‐six patients with UM were selected based on the metastatic status, and clinicopathological data were collected. Multiplex ligation‐dependent probe amplification (MLPA) was used to identify chromosomal changes. Chromosomal changes and clinicopathological data were correlated with survival and metastasis. The microRNA expression was analysed in 26 of the 36 archived UM samples. Unsupervised analysis, differential expression analysis and Cox regression analysis were performed to determine the association with metastasis and survival. Results Metastasis and metastatic death occurred in 20 patients, two patients died of other causes and one patient of unknown causes. A significant association between increasing size category (p = 0.002, log‐rank), extraocular extension (p = 0.001), chromosome 3 loss (p = 0.033) and 1p loss (p = 0.030) and development of metastases was observed. Tumour, node, metastasis (TNM) staging showed a significant association with survival (p 
ISSN:1755-375X
1755-3768
DOI:10.1111/aos.12322