A liquid-phase–blocking concanavalin A enzyme-linked immunosorbent assay for the detection of antibodies against Newcastle disease virus in serum of free-ranging pigeons

A competitive liquid-phase–blocking concanavalin A enzyme-linked immunosorbent assay (LPB-ConA-ELISA) was developed in the current study. The assay used ConA as a capture reagent, and the sera of specific pathogen–free chickens immunized with nonpurified Newcastle disease virus (NDV) suspension as d...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2013-11, Vol.25 (6), p.720-726
Main Authors: Schirato de Oliveira, Elisabete, Rodrigues Silva, Ketherson, Santos Fernando, Filipe, Costa Mello Gonçalves, Mariana, Cesário Fernandes, Camila, Monezi Borzi, Mariana, Moreira dos Santos, Romeu, Feres Tamanini, Maria de Lourdes, da Silva Montassier, Maria de Fátima, Montassier, Helio José
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - blood
Chickens
Columbidae - blood
Columbidae - virology
Concanavalin A
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
Hemagglutination Inhibition Tests - veterinary
Linear Models
Newcastle Disease - blood
Newcastle Disease - virology
Newcastle disease virus
Newcastle disease virus - isolation & purification
Reproducibility of Results
ROC Curve
Sensitivity and Specificity
Specific Pathogen-Free Organisms
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Summary:A competitive liquid-phase–blocking concanavalin A enzyme-linked immunosorbent assay (LPB-ConA-ELISA) was developed in the current study. The assay used ConA as a capture reagent, and the sera of specific pathogen–free chickens immunized with nonpurified Newcastle disease virus (NDV) suspension as detector antibodies, to detect and quantify specific antiviral antibodies in serum samples from free-ranging pigeons. The comparison between the LPB-ConA-ELISA and the hemagglutination inhibition (HI) test for the detection of antibodies in serum samples from 107 pigeons showed significant correlation between the assays (r = 0.875), a high sensitivity (100%), specificity (95.8%), accuracy (96.3%) for the ELISA, and good agreement (κ = 0.83) between the 2 assays. The results of this study suggest that the LPB-ConA-ELISA could be a useful alternative to HI test in the serodiagnosis of NDV in pigeons, or other species of birds.
ISSN:1040-6387
1943-4936
DOI:10.1177/1040638713503656