Clinical application of a molecular method based on real time RT-PCR for detection of influenza A(H1N1)v virus

Given the new diagnostic need following the pandemic caused by the A(H1N1)v virus, we evaluated the performance characteristics of Xpert® Flu assay (Cepheid). The overall sensitivity and specificity were 65.6% and 92.8%, respectively. Sensitivity and specificity for A(H1N1)v virus were 85.7% and 94....

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Bibliographic Details
Published in:The New microbiologica 2013-10, Vol.36 (4), p.405-408
Main Authors: Pierro, Anna, Gaibani, Paolo, Rossini, Giada, Landini, Maria Paola, Sambri, Vittorio
Format: Article
Language:English
Subjects:
Humans
Influenza A Virus, H1N1 Subtype - genetics
Influenza A Virus, H1N1 Subtype - isolation & purification
Influenza, Human - diagnosis
Influenza, Human - virology
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Viral - genetics
Sensitivity and Specificity
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Summary:Given the new diagnostic need following the pandemic caused by the A(H1N1)v virus, we evaluated the performance characteristics of Xpert® Flu assay (Cepheid). The overall sensitivity and specificity were 65.6% and 92.8%, respectively. Sensitivity and specificity for A(H1N1)v virus were 85.7% and 94.9%, respectively, and therefore the Xpert® Flu assay is suitable for a rapid diagnosis in critically ill patients where diagnosis is crucial for clinical management and for an appropriate public health response.
ISSN:1121-7138