Polysulfone Membranes Coated with Polymerized 3,4-Dihydroxy‑l‑phenylalanine are a Versatile and Cost-Effective Synthetic Substrate for Defined Long-Term Cultures of Human Pluripotent Stem Cells

Clinical and industrial applications of human pluripotent stem cells (hPSC) require large amounts of cells that have been expanded under defined conditions. Labor-intensive techniques and ill-defined or expensive compounds and substrates are not applicable. Here we describe a chemically defined synt...

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Bibliographic Details
Published in:Biomacromolecules 2014-06, Vol.15 (6), p.2067-2078
Main Authors: Kandasamy, Karthikeyan, Narayanan, Karthikeyan, Ni, Ming, Du, Chan, Wan, Andrew C. A, Zink, Daniele
Format: Article
Language:English
Subjects:
Animal cells
Applied sciences
Biological and medical sciences
Biotechnology
Cell Culture Techniques - economics
Cell Culture Techniques - methods
Cell Proliferation - drug effects
Cell Proliferation - physiology
Cost-Benefit Analysis - methods
Dihydroxyphenylalanine - chemistry
Dihydroxyphenylalanine - economics
Dihydroxyphenylalanine - pharmacology
Establishment of new cell lines, improvement of cultural methods, mass cultures
Eukaryotic cell cultures
Exact sciences and technology
Exchange resins and membranes
Forms of application and semi-finished materials
Fundamental and applied biological sciences. Psychology
Humans
Induced Pluripotent Stem Cells - drug effects
Induced Pluripotent Stem Cells - metabolism
Methods. Procedures. Technologies
Polymer industry, paints, wood
Polymers - chemistry
Polymers - economics
Polymers - pharmacology
Substrate Specificity - drug effects
Substrate Specificity - physiology
Sulfones - chemistry
Sulfones - economics
Technology of polymers
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Summary:Clinical and industrial applications of human pluripotent stem cells (hPSC) require large amounts of cells that have been expanded under defined conditions. Labor-intensive techniques and ill-defined or expensive compounds and substrates are not applicable. Here we describe a chemically defined synthetic substrate consisting of polysulfone (PSF) membranes coated with polymerized 3,4-dihydroxy-l-phenylalanine (DOPA). DOPA/PSF is inexpensive and can be easily produced at various shapes and sizes. DOPA/PSF supports long-term self-renewal of undifferentiated human embryonic (hESC) and human induced pluripotent stem cells (hiPSC) under defined conditions. Pluripotency is maintained for at least 10 passages. Adhesion of hPSC to DOPA/PSF is mainly mediated by a specific integrin heterodimer. Proliferation and gene expression patterns on DOPA/PSF and control substrates are comparable. Labor-intensive cultivation methods and use of serum or coating with proteins are not required. Together, these features make DOPA/PSF attractive for applications where large-scale expansion of human pluripotent stem cells under defined conditions is essential.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm5001907