Transcriptional regulation of the stress-responsive light harvesting complex genes in Chlamydomonas reinhardtii

Dissipating excess energy of light is critical for photosynthetic organisms to keep the photosynthetic apparatus functional and less harmful under stressful environmental conditions. In the green alga Chlamydomonas reinhardtii, efficient energy dissipation is achieved by a process called non-photoch...

Full description

Saved in:
Bibliographic Details
Published in:Plant and cell physiology 2014-07, Vol.55 (7), p.1304-1310
Main Authors: Maruyama, Shinichiro, Tokutsu, Ryutaro, Minagawa, Jun
Format: Article
Language:English
Subjects:
Base Sequence
Calcium Signaling - drug effects
Chlamydomonas reinhardtii - drug effects
Chlamydomonas reinhardtii - genetics
Chlamydomonas reinhardtii - physiology
Chlamydomonas reinhardtii - radiation effects
Diuron - pharmacology
Electron Transport
Enzyme Inhibitors - pharmacology
Gene Expression Regulation, Plant
Herbicides - pharmacology
Light
Light-Harvesting Protein Complexes - chemistry
Light-Harvesting Protein Complexes - genetics
Models, Molecular
Molecular Sequence Data
Multigene Family
Photosynthesis
Photosystem II Protein Complex - drug effects
Phylogeny
Protein Isoforms
Sequence Alignment
Sequence Analysis, DNA
Stress, Physiological
Sulfonamides - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Dissipating excess energy of light is critical for photosynthetic organisms to keep the photosynthetic apparatus functional and less harmful under stressful environmental conditions. In the green alga Chlamydomonas reinhardtii, efficient energy dissipation is achieved by a process called non-photochemical quenching (NPQ), in which a distinct member of light harvesting complex, LHCSR, is known to play a key role. Although it has been known that two very closely related genes (LHCSR3.1 and LHCSR3.2) encoding LHCSR3 protein and another paralogous gene LHCSR1 are present in the C. reinhardtii genome, it is unclear how these isoforms are differentiated in terms of transcriptional regulation and functionalization. Here, we show that transcripts of both of the isoforms, LHCSR3.1 and LHCSR3.2, are accumulated under high light stress. Reexamination of the genomic sequence and gene models along with survey of sequence motifs suggested that these two isoforms shared an almost identical but still distinct promoter sequence and a completely identical polypeptide sequence, with more divergent 3'-untranscribed regions. Transcriptional induction under high light condition of both isoforms was suppressed by treatment with a photosystem II inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), and a calmodulin inhibitor W7. Despite a similar response to high light, the inhibitory effects of DCMU and W7 to the LHCSR1 transcript accumulation were limited compared to LHCSR3 genes. These results suggest that the transcription of LHCSR paralogs in C. reinhardtii are regulated by light signal and differentially modulated via photosynthetic electron transfer and calmodulin-mediated calcium signaling pathway(s).
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcu068