The electrogenic cardiac sodium bicarbonate co-transporter (NBCe1) contributes to the reperfusion injury

Abstract Background Although the participation of the electrogenic sodium/bicarbonate cotransporter (NBCe1) in the recovery from an intracellular acid load is recognized, its role in ischemia–reperfusion is still unclear. Methods and results Our objective was to assess the role of NBCe1 in reperfusi...

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Bibliographic Details
Published in:Cardiovascular pathology 2014-07, Vol.23 (4), p.224-230
Main Authors: Fantinelli, Juliana C, Orlowski, Alejandro, Aiello, Ernesto A, Mosca, Susana M
Format: Article
Language:English
Subjects:
Animals
Benzamides - pharmacology
Cardiotonic Agents - pharmacology
Infarct size
Ischemia–Reperfusion
Myocardial Contraction
Myocardial Infarction - drug therapy
Myocardial Infarction - metabolism
Myocardial Infarction - pathology
Myocardial Reperfusion Injury - etiology
Myocardial Reperfusion Injury - physiopathology
Myocardial Reperfusion Injury - prevention & control
Myocardium - metabolism
Myocardium - pathology
Myocytes, Cardiac - metabolism
Myocytes, Cardiac - pathology
NBCe1
NBCe1 antibodies
Pathology
Rats
Rats, Wistar
S0859
Sodium-Bicarbonate Symporters - agonists
Sodium-Bicarbonate Symporters - antagonists & inhibitors
Sodium-Bicarbonate Symporters - metabolism
Sulfonamides - pharmacology
Ventricular Function, Left
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Summary:Abstract Background Although the participation of the electrogenic sodium/bicarbonate cotransporter (NBCe1) in the recovery from an intracellular acid load is recognized, its role in ischemia–reperfusion is still unclear. Methods and results Our objective was to assess the role of NBCe1 in reperfusion injury. We use selective functional antibodies against extracellular loop 3 (a-L3) and loop 4 (a-L4) of NBCe1. a-L3 inhibits and a-L4 stimulates NBCe1 activity. Isolated rat hearts were submitted to 40 min of coronary occlusion and 1 h of reperfusion. a-L3, a-L4 or S0859 — selective Na+ -HCO3- co-transport inhibitor — were administered during the initial 10 min of reperfusion. The infarct size (IS) was measured by triphenyltetrazolium chloride staining technique. Postischemic systolic and diastolic functions were also assessed. a-L3 and S0859 treatments decreased significantly ( P
ISSN:1054-8807
1879-1336
DOI:10.1016/j.carpath.2014.03.003