Induction of adipocyte hyperplasia in subcutaneous fat depot alleviated type 2 diabetes symptoms in obese mice

Objective The role of subcutaneous adipose tissue (SAT) in the pathogenesis of type 2 diabetes is still under controversy. In this study, the metabolic effects of inducing adipocyte hyperplasia in SAT depots in obese mice were investigated. Methods High fat diet was used to induce obesity and type 2...

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Bibliographic Details
Published in:Obesity (Silver Spring, Md.) Md.), 2014-07, Vol.22 (7), p.1623-1631
Main Authors: Lu, Qiqi, Li, Mingming, Zou, Yu, Cao, Tong
Format: Article
Language:English
Subjects:
Adipocytes
Adipocytes - metabolism
Adipocytes - pathology
Adipose tissue
Animals
Diabetes
Diabetes Mellitus, Type 2 - complications
Diabetes Mellitus, Type 2 - metabolism
Diabetes Mellitus, Type 2 - pathology
Diet
Diet, High-Fat
Hyperplasia - metabolism
Insulin Resistance
Intra-Abdominal Fat - metabolism
Intra-Abdominal Fat - pathology
Lipids
Male
Metabolism
Mice
Mice, Inbred C57BL
Mice, Obese
Mouse model
Obesity - complications
Obesity - metabolism
Obesity - pathology
Pathogenesis
Protective Factors
Studies
Subcutaneous Fat - metabolism
Subcutaneous Fat - pathology
Type 2 diabetes
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Summary:Objective The role of subcutaneous adipose tissue (SAT) in the pathogenesis of type 2 diabetes is still under controversy. In this study, the metabolic effects of inducing adipocyte hyperplasia in SAT depots in obese mice were investigated. Methods High fat diet was used to induce obesity and type 2 diabetes symptoms in C57BL6/J mice. To induce SAT expansion through hyperplasia, acellular adipogenic cocktails were injected around the SAT depots in high fat diet‐induced obese mice. Results Ten weeks after injections, significant neoadipogenesis was induced, which not only obviously expanded the volume of SATs but also significantly increased the adipocyte density within the whole SAT depots. Importantly, these mice exhibited improved glucose tolerance and insulin sensitivity (homeostatic model assessment) when compared to control group. Further studies suggested that these beneficial metabolic effects were associated with elevation of serum high‐molecular‐weight adiponectin level and reduction of ectopic lipid accumulation in liver. Conclusions These findings not only further supported the protective role of SAT in the pathogenesis of type 2 diabetes but also highlighted the importance of adipocyte hyperplasia in this protective effect.
ISSN:1930-7381
1930-739X
DOI:10.1002/oby.20705