Molecularly imprinted microspheres for the anticancer drug aminoglutethimide: Synthesis, characterization, and solid‐phase extraction applications in human urine samples

Molecularly imprinted microspheres (MIMs) for the anticancer drug aminoglutethimide (AG) were synthesized by aqueous suspension polymerization. The expected size and diameter of MIMs are controlled easily by changing one of the surfactant types, ratio of organic‐to‐water phase or stirring rate durin...

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Bibliographic Details
Published in:Journal of separation science 2014-05, Vol.37 (9-10), p.1170-1176
Main Authors: Lai, Jia‐Ping, Chen, Fang, Sun, Hui, Fan, Li, Liu, Gui‐Ling
Format: Article
Language:English
Subjects:
Aminoglutethimide
Aminoglutethimide - chemical synthesis
Aminoglutethimide - chemistry
Aminoglutethimide - urine
Analysis
Anticancer drugs
antineoplastic agents
Antineoplastic Agents - chemical synthesis
Antineoplastic Agents - chemistry
Antineoplastic Agents - urine
Aqueous suspension polymerization
Biological and medical sciences
Cancer
Chemotherapy
Chromatography
Drugs
Enrichment
Extraction
General pharmacology
high performance liquid chromatography
Human
Humans
Medical sciences
Microspheres
mixing
Molecular Imprinting
Molecularly imprinted polymers
Particle Size
Pharmacology
Pharmacology. Drug treatments
Polymerization
Separation
Solid Phase Extraction
Surface Properties
Urine
washing
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Summary:Molecularly imprinted microspheres (MIMs) for the anticancer drug aminoglutethimide (AG) were synthesized by aqueous suspension polymerization. The expected size and diameter of MIMs are controlled easily by changing one of the surfactant types, ratio of organic‐to‐water phase or stirring rate during polymerization. The obtained MIMs exhibit specific affinity toward AG with imprinting factor of 3.11 evaluated with a chromatographic model. The resultant MIMs were used as the SPE materials for the extraction of AG from human urine. A molecularly imprinted SPE (MISPE) method coupled with HPLC has been developed for the extraction and detection of AG in urine. Our results showed that most impurities from urine can be removed effectively after a washing step and the AG has been enriched effectively after MISPE operation with the recovery of >90% (n = 3). The developed MISPE–HPLC method could be used for enrichment and detection of AG in human urine.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201400008