Intact human amniotic membrane differentiated towards the chondrogenic lineage

Human amniotic membrane (hAM) represents a tissue that is well established as biomaterial in the clinics with potential for new applications in regenerative medicine. For tissue engineering (TE) strategies, cells are usually combined with inductive factors and a carrier substrate. We have previously...

Full description

Saved in:
Bibliographic Details
Published in:Cell and tissue banking 2014-06, Vol.15 (2), p.213-225
Main Authors: Lindenmair, Andrea, Nürnberger, Sylvia, Stadler, Guido, Meinl, Alexandra, Hackl, Christa, Eibl, Johann, Gabriel, Christian, Hennerbichler, Simone, Redl, Heinz, Wolbank, Susanne
Format: Article
Language:English
Subjects:
Amnion - cytology
Biomedical and Life Sciences
Biomedicine
Biopsy
Cartilage - cytology
Cell Biology
Cell culture
Cell Differentiation - physiology
Cell Lineage - physiology
Cells, Cultured
Chondrogenesis - physiology
Female
Humans
Life Sciences
Membranes
Original Paper
Placenta - cytology
Pregnancy
Stem Cells - cytology
Tissues
Transplant Surgery
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human amniotic membrane (hAM) represents a tissue that is well established as biomaterial in the clinics with potential for new applications in regenerative medicine. For tissue engineering (TE) strategies, cells are usually combined with inductive factors and a carrier substrate. We have previously recognized that hAM represents a natural, preformed sheet including highly potent stem cells. In the present approach for cartilage regeneration we have induced chondrogenesis in hAM in vitro. For this, hAM biopsies were cultured for up to 56 days under chondrogenic conditions. The induced hAM was characterized for remaining viability, glycosaminoglycan (GAG) accumulation using histochemical analysis, and a quantitative assay. Collagen I, II and X was immunohistochemically determined and cartilage-specific mRNA expression of (sex determining region Y-) box 9, cartilage oligomeric matrix protein (COMP), aggrecan (AGC1), versican (CSPG2), COL1A1, COL9A2, melanoma inhibitory activity (MIA), and cartilage-linking protein 1 (CRTL1) analyzed by quantitative real-time polymerase chain reaction. Human AM was successfully induced to accumulate GAG, as demonstrated by Alcianblue staining and a significant ( p  
ISSN:1389-9333
1573-6814
DOI:10.1007/s10561-014-9454-9