An in vitro system for studying the effects of ozone on mammalian cell cultures and viruses

A unique in vitro system was developed for exposing mammalian cell cultures, viruses, or both to ozone under conditions mimicking those of the respiratory tract. The system used borosilicate glass roller culture bottles equipped with specially designed caps to permit the flow of humidified gas (ozon...

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Bibliographic Details
Published in:Environmental research 1982-04, Vol.27 (2), p.466-475
Main Authors: Bolton, David C., Tarkington, Brian K., Zee, Yuan Chung, Osebold, John W.
Format: Article
Language:English
Subjects:
cell membranes
Cells, Cultured
Culture Media
Humans
mammalian cells
membrane proteins
ozone
Ozone - metabolism
Ozone - toxicity
Respiratory System - drug effects
Time Factors
viruses
Viruses - drug effects
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Summary:A unique in vitro system was developed for exposing mammalian cell cultures, viruses, or both to ozone under conditions mimicking those of the respiratory tract. The system used borosilicate glass roller culture bottles equipped with specially designed caps to permit the flow of humidified gas (ozone or filtered air) through the rotating vessels. The system was designed to allow two test cultures and one control culture to be simultaneously exposed to different precisely defined concentrations of ozone. The input and exhaust concentrations of ozone were sequentially monitored with an ultraviolet photometric ozone analyzer. The system was used to determine the reactivity of ozone with several tissue culture media at different flow rates. The reaction rate of ozone with media was shown to be a function of the input concentration and increased as the gas flow rate was increased. Input ozone concentrations measured during 48-hr test exposures remained stable, yielding standard deviations of less than 4%. Exposure of Madin-Darby bovine kidney cells to ozone concentrations of 0.16 and 0.64 ppm for 24 hr resulted in a slight but significant decrease in the synthesis of RNA as measured by the incorporation of [ 3H]uridine into TCA-precipitable material. The synthesis of protein and DNA was not significantly affected by identical treatments. Vesicular stomatitis virus exposed to ozone at concentrations of 0.16 and 0.64 ppm for 12 hr showed marked loss of biological function when compared to identical controls exposed to filtered air. The feasibility of using enveloped viruses as a model for investigation of ozone-induced damage to cellular membranes and membrane proteins is discussed.
ISSN:0013-9351
1096-0953
DOI:10.1016/0013-9351(82)90101-3