Study of the Spatial Distribution of Mercury in Roots of Vetiver Grass (Chrysopogon zizanioides) by Micro-Pixe Spectrometry

Localization of Hg in root tissues of vetiver grass (Chrysopogon zizanioides) was investigated by micro-Proton Induced X-ray Emission (PIXE) spectrometry to gain a better understanding of Hg uptake and its translocation to the aerial plant parts. Tillers of C. zizanioides were grown in a hydroponic...

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Bibliographic Details
Published in:International journal of phytoremediation 2014, Vol.16 (11), p.1170-1182
Main Authors: Lomonte, Cristina, Wang, Yaodong, Doronila, Augustine, Gregory, David, Baker, Alan J. M, Siegele, Rainer, Kolev, Spas D
Format: Article
Language:English
Subjects:
aerial parts
Aerials
assisted-phytoextraction
Biodegradation, Environmental
Biological Transport
Bioremediation
Chelating
Chelating Agents
Chrysopogon - cytology
Chrysopogon - metabolism
Chrysopogon zizanioides
Grasses
Hydroponics
Mercury
Mercury (metal)
Mercury - analysis
Mercury - metabolism
micro-PIXE spectrometry
phytoremediation
Plant Components, Aerial - cytology
Plant Components, Aerial - metabolism
Plant Leaves - cytology
Plant Leaves - metabolism
Plant Roots - cytology
Plant Roots - metabolism
Plant Vascular Bundle - cytology
Plant Vascular Bundle - metabolism
Position (location)
Roots
Scientific imaging
Spectrometry
Spectrometry, X-Ray Emission - methods
Spectroscopy
suberin
tillers
X-radiation
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Summary:Localization of Hg in root tissues of vetiver grass (Chrysopogon zizanioides) was investigated by micro-Proton Induced X-ray Emission (PIXE) spectrometry to gain a better understanding of Hg uptake and its translocation to the aerial plant parts. Tillers of C. zizanioides were grown in a hydroponic culture for 3 weeks under controlled conditions and then exposed to Hg for 10 days with or without the addition of the chelators (NH ₄) ₂S ₂O ₃ or KI. These treatments were used to study the effects of these chelators on localization of Hg in the root tissues to allow better understanding of Hg uptake during its assisted-phytoextraction. Qualitative elemental micro-PIXE analysis revealed that Hg was mainly localized in the root epidermis and exodermis, tissues containing suberin in all Hg treatments. Hg at trace levels was localized in the vascular bundle when plants were treated with a mercury solution only. However, higher Hg concentrations were found when the solution also contained (NH ₄) ₂S ₂O ₃ or KI. This finding is consistent with the observed increase in Hg translocation to the aerial parts of the plants in the case of chemically induced Hg phytoextraction.
ISSN:1549-7879
1522-6514
1549-7879
DOI:10.1080/15226514.2013.821453