Growth of a Catharanthus roseus cell suspension culture in a modified chemostat under glucose-limiting conditions

A system for the continuous cultivation of plant cells has been developed, based on a commercially available 3-1 turbine-stirred fermentor. A special device was constructed to provide for homogeneous effluent from the culture at low dilution rates. Two steady states with Catharanthus roseus cells gr...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1989-03, Vol.30 (3), p.270-275
Main Authors: Gulik, W.M. van, Meijer, J.J, Hoopen, H.J.G. ten, Luyben, K.C.A.M, Libbenga, K.R
Format: Article
Language:English
Subjects:
Biological and medical sciences
biomass
Biotechnology
Catharanthus roseus
cell culture
Cell cultures. Hybridization. Fusion
chemostats
Eukaryotic cell cultures
fermenters
Fundamental and applied biological sciences. Psychology
glucose
growth rate
Methods. Procedures. Technologies
Miscellaneous
Molecular and cellular biology
oxygen consumption
Plant cells and fungal cells
suspension culture
suspensions
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Summary:A system for the continuous cultivation of plant cells has been developed, based on a commercially available 3-1 turbine-stirred fermentor. A special device was constructed to provide for homogeneous effluent from the culture at low dilution rates. Two steady states with Catharanthus roseus cells growing under glucose limitation are described with respect to biomass yield on the carbon and energy source glucose, specific oxygen consumption, specific carbon dioxide production and (by) product formation. From a carbon balance for each steady state it is shown that the flow of carbon to the culture (as glucose) practically equalled the flow of carbon from the culture (as biomass, carbon dioxide and (by) products). Biomass yields on glucose were 0.31 g/g and 0.35 g/g at dilution rates of 0.0060 1/h and 0.0081 1/h respectively.
ISSN:0175-7598
1432-0614
DOI:10.1007/BF00256217