Improved separation of basic peptides in anion-exchange chromatography

A method is described for the separation of peptides on anion-exchange-resin columns with particular emphasis being given to the resolution of basic arginine and basic lysine peptides. The addition of N-methylpiperidine, p K a 10.08, to the solvent systems used either with stepwise or gradient separ...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 1981-01, Vol.215, p.109-114
Main Authors: Malmström, Betty M., Nyman, Per-Olof, Strid, Lars
Format: Article
Language:English
Subjects:
anion-exchange chromatography
methodology
peptides
purification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A method is described for the separation of peptides on anion-exchange-resin columns with particular emphasis being given to the resolution of basic arginine and basic lysine peptides. The addition of N-methylpiperidine, p K a 10.08, to the solvent systems used either with stepwise or gradient separations improves the resolution in the high pH range compared to methods previously used.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)81391-6