Molecular evidence for nitrite-dependent anaerobic methane-oxidising bacteria in the Jiaojiang Estuary of the East Sea (China)

Nitrite-dependent anaerobic methane oxidation (N-DAMO) is a recently discovered process linking the global carbon and nitrogen cycles. This process was reported to be mediated by “ Candidatus Methylomirabilis oxyfera ”. To date, M. oxyfera -like bacteria have been detected in a limited number of fre...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2014-06, Vol.98 (11), p.5029-5038
Main Authors: Li-dong, Shen, Qun, Zhu, Shuai, Liu, Ping, Du, Jiang-ning, Zeng, Dong-qing, Cheng, Xiang-yang, Xu, Ping, Zheng, Bao-lan, Hu
Format: Article
Language:English
Subjects:
Anaerobic bacteria
Anaerobiosis
Analysis
Applied Genetics and Molecular Biotechnology
Bacteria
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bacteria - metabolism
Bacterial Proteins - genetics
Biodiversity
Biomedical and Life Sciences
Biotechnology
Carbon
China
Cluster Analysis
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Ecosystems
Environmental aspects
Environmental conditions
Estuaries
Estuarine ecology
Genes
Genetic aspects
Geologic Sediments - microbiology
Laboratories
Life Sciences
Methane
Methane - metabolism
Microbial Genetics and Genomics
Microbiology
Molecular Sequence Data
Nitrites - metabolism
Oxidation
Oxidation-Reduction
Phylogeny
Properties
Rivers
RNA, Ribosomal, 16S - genetics
Seawater
Sediments
Sequence Analysis, DNA
Studies
Water treatment
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Summary:Nitrite-dependent anaerobic methane oxidation (N-DAMO) is a recently discovered process linking the global carbon and nitrogen cycles. This process was reported to be mediated by “ Candidatus Methylomirabilis oxyfera ”. To date, M. oxyfera -like bacteria have been detected in a limited number of freshwater habitats, but whether these bacteria occur in estuarine habitats is currently unknown. In this study, the distribution, diversity and abundance of M. oxyfera -like bacteria were studied in the sediment of the Jiaojiang Estuary of the East Sea (China). Both the 16S ribosomal RNA (rRNA) and pmoA genes confirmed the occurrence of M. oxyfera -like bacteria in the examined estuary. The recovered 16S rRNA gene sequences showed 91.5–97.2 % identity to the 16S rRNA gene of M. oxyfera , and the recovered pmoA gene sequences showed 85.1–95.4 % identity to the pmoA gene of M. oxyfera . Quantitative PCR further confirmed the occurrence of M. oxyfera -like bacteria in this estuary, with the abundance varying from 5.80 ± 0.28 × 10 4 to 8.35 ± 0.52 × 10 7  copies g (dry weight) −1 . Correlation analysis indicated that the sediment organic content was the most important factor affecting the distribution of M. oxyfera -like bacterial communities in the examined sediments among the environmental factors investigated. This study demonstrated for the first time the existence of M. oxyfera -like bacteria in an estuarine environment and showed the correlations between the distribution of these bacteria and the estuarine environmental conditions.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-014-5556-3