Involvement of satellite I noncoding RNA in regulation of chromosome segregation

Human centromeres consist of repetitive sequences from which satellite I noncoding RNAs are transcribed. We found that knockdown of satellite I RNA causes abnormal chromosome segregation and generation of nuclei with a grape‐shape phenotype. Co‐immunoprecipitation experiments showed that satellite I...

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Bibliographic Details
Published in:Genes to cells : devoted to molecular & cellular mechanisms 2014-06, Vol.19 (6), p.528-538
Main Authors: Ideue, Takashi, Cho, Yukiko, Nishimura, Kanako, Tani, Tokio
Format: Article
Language:English
Subjects:
Aurora Kinase B - genetics
Aurora Kinase B - metabolism
Cell Nucleus - genetics
Cell Nucleus - metabolism
Cell Nucleus Division
Centromere - genetics
Centromere - metabolism
Chromatin
Chromosomal Proteins, Non-Histone - genetics
Chromosomal Proteins, Non-Histone - metabolism
Chromosome Segregation - physiology
Chromosomes
HeLa Cells
Humans
Interphase
Kinases
RNA, Untranslated - metabolism
Satellites
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Summary:Human centromeres consist of repetitive sequences from which satellite I noncoding RNAs are transcribed. We found that knockdown of satellite I RNA causes abnormal chromosome segregation and generation of nuclei with a grape‐shape phenotype. Co‐immunoprecipitation experiments showed that satellite I RNA associates with Aurora B, a component of the chromosome passenger complex (CPC) regulating proper attachment of microtubules to kinetochores, in mitotic HeLa cells. Satellite I RNA was also shown to associate with INCENP, another component of the CPC. In addition, depletion of satellite I RNA resulted in up‐regulation of kinase activity of Aurora B and delocalization of the CPC from the centromere region. These results suggest that satellite I RNA is involved in chromosome segregation through controlling activity and centromeric localization of Aurora B kinase.
ISSN:1356-9597
1365-2443
DOI:10.1111/gtc.12149