Embryonic development of GABAergic signaling in the mouse spinal trigeminal nucleus interpolaris

•Developmental changes in GABAergic transmission were investigated in the mouse SpVi.•The SpVi may first receive GABAergic projection fibers from extra-nuclear area.•GABAergic neurons were localized after E15 and may form synapses after E17.•GABA action may shift from excitatory to inhibitory betwee...

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Bibliographic Details
Published in:Neuroscience letters 2014-04, Vol.566, p.221-225
Main Authors: Kin, Hidemichi, Kim, Jeongtae, Shimizu-Okabe, Chigusa, Okabe, Akihito, Takayama, Chitoshi
Format: Article
Language:English
Subjects:
Animals
Biomarkers - metabolism
Embryonic Development
GABAergic Neurons - metabolism
GABAergic synapse
gamma-Aminobutyric Acid - metabolism
Glutamate Decarboxylase - metabolism
Glutamic acid decarboxylase (GAD)
K Cl- Cotransporters
Mice, Inbred C57BL
Potassium chloride co-transporter 2 (KCC2)
Signal Transduction
Symporters - metabolism
Synapses - metabolism
Trigeminal Nucleus, Spinal - embryology
Trigeminal Nucleus, Spinal - metabolism
Vesicular GABA transporter (VGAT)
Vesicular Inhibitory Amino Acid Transport Proteins - metabolism
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Summary:•Developmental changes in GABAergic transmission were investigated in the mouse SpVi.•The SpVi may first receive GABAergic projection fibers from extra-nuclear area.•GABAergic neurons were localized after E15 and may form synapses after E17.•GABA action may shift from excitatory to inhibitory between E13 and E17. In the mature central nervous system, γ-amino butyric acid (GABA) is an inhibitory neurotransmitter, whereas during development, GABA induces depolarization. To examine the embryonic development of GABAergic transmission in the mouse spinal trigeminal nucleus interpolaris (SpVi), which receives sensory input from the face and is important in survival of rodents, we performed immunohistochemistry for three related molecules: glutamic acid decarboxylase (GAD), a marker of GABAergic neurons; vesicular GABA transporter (VGAT), a marker of GABAergic and glycinergic vesicles; and potassium chloride co-transporter 2 (KCC2), which shifts GABA action from excitatory to inhibitory. GAD-positive longitudinal projection fibers, where VGAT-positive dots were localized, were clearly discernible until embryonic day (E)17, and were markedly decreased in number on postnatal day 0. GAD-positive neurons were detected after E15, and GAD- and VGAT-positive axon varicosities were observed after E17. KCC2 immunolabeling was first localized in the dendrites and cell bodies of several neurons in the lateral part of the SpVi on E13 and throughout the nucleus on E17. These results suggest that the SpVi may first receive GABAergic projection fibers from extra-nuclear area before birth, and GABAergic interneurons may form synapses within the SpVi after E17. In addition, GABA action may gradually shift from excitatory to inhibitory between E13 and E17.
ISSN:0304-3940
1872-7972
DOI:10.1016/j.neulet.2014.02.057