Production of Novel “Functional Oil” Rich in Diglycerides and Phytosterol Esters with “One-Pot” Enzymatic Transesterification

Diglycerides and phytosterol esters are two important functional lipids. Phytosterol esters mixed with dietary diglyceride could not only influence body weight but also prevent or reverse insulin resistance and hyperlipidemia. In this study, a kind of novel “functional oil” rich in both diglycerides...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2014-06, Vol.62 (22), p.5142-5148
Main Authors: Zheng, Ming-Ming, Huang, Qing, Huang, Feng-Hong, Guo, Ping-Mei, Xiang, Xia, Deng, Qian-Chun, Li, Wen-Lin, Wan, Chu-Yun, Zheng, Chang
Format: Article
Language:English
Subjects:
acid value
biocatalysts
body weight
Candida - enzymology
Candida rugosa
crosslinking
diacylglycerols
Diglycerides - chemistry
Enzyme Stability
Enzymes, Immobilized - chemistry
Esterification
Esters - chemistry
fatty acid composition
Functional Food - analysis
functional foods
Fungal Proteins - chemistry
hexane
hydrophobic bonding
hyperlipidemia
insulin resistance
Lipase - chemistry
oils
peroxide value
phytosterols
Phytosterols - chemistry
Plant Oils - chemistry
polystyrenes
Sunflower Oil
thermal stability
transesterification
triacylglycerols
Triglycerides - chemistry
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Summary:Diglycerides and phytosterol esters are two important functional lipids. Phytosterol esters mixed with dietary diglyceride could not only influence body weight but also prevent or reverse insulin resistance and hyperlipidemia. In this study, a kind of novel “functional oil” rich in both diglycerides and phytosterol esters was prepared with “one-pot” enzymatic transesterification. First, lipase AYS (Candida rugosa) was immobilized on the porous cross-linked polystyrene resin beads (NKA) via hydrophobic interaction. The resulting immobilized AYS showed much better transesterification activity and thermal stability to freeways. On the basis of the excellent biocatalyst prepared, a method for high-efficiency enzymatic esterification of phytosterols with different triglycerides to produce corresponding functional oils rich in both diglycerides and phytosterol esters was developed. Four functional oils rich in both diglycerides and phytosterol esters with conversions >92.1% and controllable fatty acid composition were obtained under the optimized conditions: 80 mmol/L phytosterols, 160 mmol/L triglycerides, and 25 mg/mL AYS@NKA at 180 rpm and 50 °C for 12 h in hexane. The prepared functional oil possessed low acid value (≤1.0 mgKOH/g), peroxide value (≤2.1 mmol/kg), and conjugated diene value (≤1.96 mmol/kg) and high diglyceride and phytosterol ester contents (≥10.4 and ≥20.2%, respectively). All of the characteristics favored the wide application of the functional oil in different fields of functional food.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf500744n