Characterization and genotoxicity evaluation of particulate matter collected from industrial atmosphere in Tamil Nadu State, India

Ambient particulate matter (PM) collected in the vicinity of five industries (Cement, Chemical, Thermal power plant, Sponge-iron and Steel) in Tamil Nadu state, India was characterized for size distribution, metals and polycyclic aromatic hydrocarbons (PAHs) content. Genotoxicity of PM and organic m...

Full description

Saved in:
Bibliographic Details
Published in:Journal of hazardous materials 2014-06, Vol.274, p.392-398
Main Authors: SENTHILKUMAR, S, MANJU, A, MUTHUSELVAM, P, SHALINI, D, INDHUMATHI, V, KALAISELVI, K, PALANIVEL, M, CHANDRASEKAR, P. P, RAJAGURU, P
Format: Article
Language:English
Subjects:
Air Pollutants - analysis
Air Pollutants - chemistry
Air Pollutants - toxicity
Antioxidants - pharmacology
Applied sciences
Atmospheric pollution
Cell Line, Tumor
Cell Survival - drug effects
Comet Assay
DNA Damage
Environmental Monitoring
Exact sciences and technology
Hep G2 Cells
Humans
India
Industry
Metals, Heavy - analysis
Metals, Heavy - chemistry
Metals, Heavy - toxicity
Particle Size
Particulate Matter - analysis
Particulate Matter - chemistry
Particulate Matter - toxicity
Pollution
Polycyclic Aromatic Hydrocarbons - analysis
Polycyclic Aromatic Hydrocarbons - chemistry
Polycyclic Aromatic Hydrocarbons - toxicity
Quercetin - pharmacology
Vitamins - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ambient particulate matter (PM) collected in the vicinity of five industries (Cement, Chemical, Thermal power plant, Sponge-iron and Steel) in Tamil Nadu state, India was characterized for size distribution, metals and polycyclic aromatic hydrocarbons (PAHs) content. Genotoxicity of PM and organic matter (OM) extracted from PM was measured in human lung cancer cell-line, A549 and in human liver carcinoma cell-line, HepG2, respectively, using the comet assay. PM values varied from 57.0 μg/m(3) of air at Cement industry upstream to 561.0 μg/m(3) of air at Sponge iron industry downstream samples. Their metal content varied from 5.758 μg/m(3) of air at Chemical industry to 46.144 μg/m(3) of air at Sponge iron industry and PAH concentration varied from 0.5 ng/m(3) air in upstream Thermal power plant to 3302.4 ng/m(3) air in downstream Sponge iron industry samples. While all PM samples induced DNA strand breaks at higher dose levels, downstream samples of Steel and Sponge iron industries which contained relatively higher concentrations of PAHs and metals and exhibited higher levels of pro-oxidant activity as measured by DTT activity induced significantly higher levels of DNA damage in HepG2 and A549 cells. Pretreatment of A549 cells with vitamin C or quercetin significantly reduced PM induced DNA strand breaks.
ISSN:0304-3894
1873-3336
DOI:10.1016/j.jhazmat.2014.04.017