Atypical embryo phenotypes identified by time-lapse microscopy: high prevalence and association with embryo development

Objective To characterize atypical dynamic embryo phenotypes identified by time-lapse microscopy, evaluate their prevalence, and determine their association with embryo development. Design Retrospective multicenter cohort study. Setting Five IVF clinics in the United States. Patient(s) Sixty-seven w...

Full description

Saved in:
Bibliographic Details
Published in:Fertility and sterility 2014-06, Vol.101 (6), p.1637-1648.e5
Main Authors: Athayde Wirka, Kelly, M.S, Chen, Alice A., Ph.D, Conaghan, Joe, Ph.D, Ivani, Kristen, Ph.D, Gvakharia, Marina, M.D., Ph.D, Behr, Barry, Ph.D, Suraj, Vaishali, M.S, Tan, Lei, Ph.D, Shen, Shehua, M.D
Format: Article
Language:English
Subjects:
abnormal embryo development
Adult
Atypical phenotype
Blastocyst - pathology
California
Cleavage Stage, Ovum
Cytokinesis
Embryo Culture Techniques
embryo selection
embryo viability assessment
Embryo, Mammalian - pathology
Embryonic Development
Female
Fertilization in Vitro
Humans
Internal Medicine
Male
Microscopy, Video
Obstetrics and Gynecology
Phenotype
Retrospective Studies
Sperm Injections, Intracytoplasmic
Time Factors
Time-Lapse Imaging
time-lapse microscopy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective To characterize atypical dynamic embryo phenotypes identified by time-lapse microscopy, evaluate their prevalence, and determine their association with embryo development. Design Retrospective multicenter cohort study. Setting Five IVF clinics in the United States. Patient(s) Sixty-seven women undergoing IVF treatment with 651 embryos. Intervention(s) Embryo videos were retrospectively analyzed for atypical phenotypes. Main Outcome Measure(s) Identification of four groups of atypical embryo phenotypes: abnormal syngamy (AS), abnormal first cytokinesis (A1cyt ), abnormal cleavage (AC), and chaotic cleavage (CC). Prevalence and association with embryo morphology and development potential were evaluated. Result(s) A high prevalence of atypical phenotypes was observed among embryos: AS 25.1% (163/649), A1cyt 31.0% (195/639), AC 18% (115/639) and CC 15% (96/639). A high percentage of embryos with atypical phenotype(s) had good quality on day 3 (overall grade good or fair): AS 78.6% (70/89); A1cyt 79.7% (94/119), AC 86.4% (70/81), and CC 35.2% (19/54), but the blastocyst formation rates for these embryos were significantly lower compared with their respective control groups: AS 21.5% vs. 44.9%, A1cyt 21.7% vs. 44.6%, AC 11.7% vs. 43.1%, and CC 14.0% vs. 42.3%. Conclusion(s) Embryos exhibiting atypical phenotypes are highly prevalent in human embryos and show significantly lower developmental potential than control embryos. Clinical Trial Registration Number NCT01369446.
ISSN:0015-0282
1556-5653
DOI:10.1016/j.fertnstert.2014.02.050