Spectroscopic studies on the interaction of bovine serum albumin with Al2O3 nanoparticles

Since the nanoparticle usage in the biomedical field is increasing, it is necessary to understand their interaction with the biomolecules, such as proteins. The current study primarily investigates the interaction of BSA with the Al2O3 nanoparticles by various spectroscopic techniques. The experimen...

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Bibliographic Details
Published in:Journal of luminescence 2014-01, Vol.145, p.859-865
Main Authors: Rajeshwari, A., Pakrashi, Sunandan, Dalai, Swayamprava, Madhumita, Iswarya, V., Chandrasekaran, N., Mukherjee, Amitava
Format: Article
Language:English
Subjects:
Al2O3
Aluminum oxide
Biomolecules
Bovine serum albumin
Condensed matter: electronic structure, electrical, magnetic, and optical properties
Cross-disciplinary physics: materials science
rheology
Dichroism
Exact sciences and technology
Ground state
Ground state complex
Materials science
Nanoparticles
Nanopowders
Nanoscale materials and structures: fabrication and characterization
Optical properties and condensed-matter spectroscopy and other interactions of matter with particles and radiation
Photoluminescence
Physics
Secondary structure
Spectroscopic analysis
Spectroscopy
Surface chemistry
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Summary:Since the nanoparticle usage in the biomedical field is increasing, it is necessary to understand their interaction with the biomolecules, such as proteins. The current study primarily investigates the interaction of BSA with the Al2O3 nanoparticles by various spectroscopic techniques. The experiments were carried out in three different experimental matrices, i.e. the distilled de-ionized water, the phosphate buffer and the saline media. The enhanced absorbance observed by UV–visible and fluorescence spectroscopy suggested the probable formation of a ground state complex of the type BSA–Al2O3. The apparent association constant (Kapp), calculated based on the spectral changes due to the association of BSA with Al2O3 NPs, was found to be higher in an aqueous system (pH 4.47) as compared to the other two matrices, suggesting the maximum interaction between BSA and Al2O3. The particle size analysis of Al2O3 in aqueous suspension demonstrated the possibility of BSA adsorption onto the NP surface. The FT-IR and the circular dichroism (CD) studies indicated that the Al2O3 NPs induced the structural changes in the BSA secondary structure, especially α-helix. •BSA interaction with Al2O3 caused aggregation of NPs.•Enhanced absorption due to the ground state complex.•Non significant quenching effect due to absence of corona formation.•Conformation change in BSA.
ISSN:0022-2313
1872-7883
DOI:10.1016/j.jlumin.2013.08.073