Pathogenicity and transmission of H5N1 avian influenza viruses in different birds

In this study, we selected three H5N1 highly pathogenic avian influenza viruses (HPAIVs), A/Goose/Guangdong/1/1996 (clades 0), A/Duck/Guangdong/E35/2012 (clade 2.3.2.1) and A/Chicken/Henan/B30/2012 (clade 7.2) isolated from different birds in China, to investigate the pathogenicity and transmission...

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Bibliographic Details
Published in:Veterinary microbiology 2014-01, Vol.168 (1), p.50-59
Main Authors: Yuan, Runyu, Cui, Jin, Zhang, Shuo, Cao, Lan, Liu, Xiaoke, Kang, Yinfeng, Song, Yafen, Gong, Lang, Jiao, Peirong, Liao, Ming
Format: Article
Language:English
Subjects:
Animals
Avian influenza virus
Chickens - virology
China
Ducks - virology
Geese - virology
Genes, Viral - genetics
H5N1
Influenza A Virus, H5N1 Subtype - classification
Influenza A Virus, H5N1 Subtype - genetics
Influenza A Virus, H5N1 Subtype - isolation & purification
Influenza A Virus, H5N1 Subtype - pathogenicity
Influenza in Birds - mortality
Influenza in Birds - transmission
Influenza in Birds - virology
Pathogenicity
Phylogeny
Transmission
Virulence - genetics
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Summary:In this study, we selected three H5N1 highly pathogenic avian influenza viruses (HPAIVs), A/Goose/Guangdong/1/1996 (clades 0), A/Duck/Guangdong/E35/2012 (clade 2.3.2.1) and A/Chicken/Henan/B30/2012 (clade 7.2) isolated from different birds in China, to investigate the pathogenicity and transmission of the viruses in terrestrial birds and waterfowl. To observe the replication and shedding of the H5N1 HPAIVs in birds, the chickens were inoculated intranasally with 106 EID50 of GSGD/1/96, 103 EID50 of DkE35 and CkB30, and the ducks and geese were inoculated intranasally with 106 EID50 of each virus. Meanwhile, the naive contact groups were set up to detect the transmission of the viruses in tested birds. Our results showed that DkE35 was highly pathogenic to chickens and geese, but not fatal to ducks. It could be detected from all the tested organs, oropharyngeal and cloacal swabs, and could transmit to the naive contact birds. GSGD/1/96 could infect chickens, ducks and geese, but only caused death in chickens. It could transmit to the chickens and ducks, but was not transmittable to geese. CkB30 was highly pathogenic to chickens, low pathogenic to ducks and not pathogenic to geese. It could be transmitted to the naive contact chickens, but not to the ducks or geese. Our findings suggested that H5N1 HPAIVs from different birds show different host ranges and tissue tropisms. Therefore, we should enhance serological and virological surveillance of H5N1 HPAIVs, and pay more attention to the pathogenic and antigenic evolution of these viruses.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2013.10.013