Effects of bisphenol A and 4-nonylphenol on cellular responses through the different induction of LPA receptors in liver epithelial WB-F344 cells

Abstract Lysophosphatidic acid (LPA) signaling via G protein-coupled transmembrane LPA receptors (LPA1 to LPA6) mediates a variety of cellular functions, including cell proliferation, migration, morphogenesis, and differentiation. Recently, we demonstrated that the different induction of LPA recepto...

Full description

Saved in:
Bibliographic Details
Published in:Journal of receptors and signal transduction 2014-06, Vol.34 (3), p.201-204
Main Authors: Dong, Yan, Araki, Mutsumi, Hirane, Miku, Tanabe, Eriko, Fukushima, Nobuyuki, Tsujiuchi, Toshifumi
Format: Article
Language:English
Subjects:
4-nonylphenol
Animals
Benzhydryl Compounds - administration & dosage
bisphenol A
Cell Line
cell motility
Cell Movement - drug effects
Cell Movement - physiology
Cell Proliferation - drug effects
Cell Proliferation - physiology
Dose-Response Relationship, Drug
Endocrine Disruptors - administration & dosage
Epithelial Cells - cytology
Epithelial Cells - drug effects
Epithelial Cells - physiology
Liver - cytology
Liver - drug effects
Liver - physiology
LPA
LPA receptor
Lysophospholipids - metabolism
Phenols - administration & dosage
Rats
Receptors, Lysophosphatidic Acid - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Lysophosphatidic acid (LPA) signaling via G protein-coupled transmembrane LPA receptors (LPA1 to LPA6) mediates a variety of cellular functions, including cell proliferation, migration, morphogenesis, and differentiation. Recently, we demonstrated that the different induction of LPA receptors by estrogens regulates cell motile activity of rat liver epithelial WB-F344 cells. In the present study, to assess whether endocrine disruptors (EDs) are involved in cellular functions through LPA signaling, we measured cell motile activity and LPA receptor expressions in WB-F344 cells treated with bisphenol A (BPA) and 4-nonylphenol (4-NP). Using quantitative real time RT-PCR analysis, the Lpar1 expression was elevated in BPA-treated cells, whereas the Lpar3 expression was decreased. In contrast, 4-NP increased the Lpar3 expression, but not the Lpar1 and Lpar2. For cell motility assay with a Cell Culture Insert, cell motile activity of BPA-treated cells was significantly lower than that of untreated cells. In contrast, 4-NP markedly enhanced cell motile activity. The effects of BPA and 4-NP on cell motility were inhibited by the Lpar1 or Lpar3 knockdown. These results suggest that BPA and 4-NP may regulate cell motile activity through the different induction of LPA receptors in WB-F344 cells.
ISSN:1079-9893
1532-4281
DOI:10.3109/10799893.2013.876040