The purification of cytokinin oxidase from Zea mays kernels

Cytokinin oxidase has been purified to apparent homogeneity from Zea mays kernels as indicated by a single protein staining spot on a 2-D, IEF/SDS PAGE gel. Polyclonal antibodies raised to this protein were able to precipitate cytokinin oxidase activity from a highly active, partially purified (QAE-...

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Bibliographic Details
Published in:Phytochemistry (Oxford) 1989, Vol.28 (5), p.1313-1319
Main Authors: Burch, Lindsay R., Horgan, Roger
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
chemical constituents of plants
cytokinin metabolism
cytokinin oxidase
cytokinins
enzyme activity
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Gramineae
Oxidoreductases
proteins
purification
seeds
sweetcorn kernels
Zea mays
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Summary:Cytokinin oxidase has been purified to apparent homogeneity from Zea mays kernels as indicated by a single protein staining spot on a 2-D, IEF/SDS PAGE gel. Polyclonal antibodies raised to this protein were able to precipitate cytokinin oxidase activity from a highly active, partially purified (QAE-Sephadex) preparation in the presence of fixed Staphylococcus aureus cells. The polyclonal antibodies raised to cytokinin oxidase from Z. mays crossreact with a similar protein in partially purified cytokinin oxidase preparations from dried wheat seeds.
ISSN:0031-9422
1873-3700
DOI:10.1016/S0031-9422(00)97737-9