A novel in vitro assay for murine haematopoietic stem cells

Study of the biology of haematopoietic stem cells is crucially dependent on the availability of suitable in vitro assays. Existing assays have suffered from the fact that they detect small subcompartments of the total stem cell compartment. The authors describe an in vitro assay which shows macrosco...

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Bibliographic Details
Published in:British Journal of Cancer. 1988 1988, Vol.58, p.36-40
Main Authors: ECKMANN, L, FRESHNEY, M, WRIGHT, E. G, SPROUL, A, WILKIE, N, PRAGNELL, I. B
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell differentiation, maturation, development, hematopoiesis
Cell physiology
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
stem cells
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Summary:Study of the biology of haematopoietic stem cells is crucially dependent on the availability of suitable in vitro assays. Existing assays have suffered from the fact that they detect small subcompartments of the total stem cell compartment. The authors describe an in vitro assay which shows macroscopic colony formation and limited self-renewal capacity in vitro. The detected cell (CFU-A) has a low cycling status in normal bone marrow (NBM) and responds to known stem cell regulators. The data indicate that the authors' assay detects a high proportion of haematopoietic stem cells in vitro. This will facilitate experiments on stem cell behaviour which have previously been difficult to conduct.
ISSN:0007-0920
1532-1827