The Ycf54 protein is part of the membrane component of Mg‐protoporphyrin IX monomethyl ester cyclase from barley (Hordeum vulgareL.)

The biosynthesis of chlorophyll has been demonstrated to require an extensive set of enzymes, the initial stages of which are shared with the synthesis of heme. Of these enzymes, the most enigmatic is the Mg‐protoporphyrin IX monomethyl ester cyclase (EC 1.14.13.81). This enzyme requires components...

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Bibliographic Details
Published in:	The FEBS journal 2014-05, Vol.281 (10), p.2377-2386
Main Authors:	Bollivar, David, Braumann, Ilka, Berendt, Kasper, Gough, Simon P., Hansson, Mats
Format:	Article
Language:	eng
Subjects:	Barley Biosynthesis chlorophyll cyclase Enzymes etioplast Genes, Plant Hordeum - genetics Hordeum - metabolism isocyclic ring Membranes Mg‐protoporphyrin monomethyl ester Mutation Oxygenases - chemistry Oxygenases - genetics Oxygenases - metabolism Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Plastids - metabolism Proteins Protoporphyrins - metabolism
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Summary:	The biosynthesis of chlorophyll has been demonstrated to require an extensive set of enzymes, the initial stages of which are shared with the synthesis of heme. Of these enzymes, the most enigmatic is the Mg‐protoporphyrin IX monomethyl ester cyclase (EC 1.14.13.81). This enzyme requires components found associated with the plastid membrane and the plastid soluble fraction. One of the components, XanL, is found associated with the membrane and another protein, Ycf54, has recently been identified based upon association with XanL. This study describes a deeper analysis of the role of Ycf54 in the enzyme and the localization of the protein in barley plastids. The results clearly demonstrate a strong association of Ycf54 with XanL, absence of Ycf54 from soluble fractions necessary for activity and more evidence for a third membrane localized component of the enzyme. Structured digital Ycf54 and Ycf54 bind by molecular sieving (1, 2) The enzyme Mg‐protoporphyrin IX monomethyl ester cyclase (EC 1.14.13.81) plays a central role in the biosynthesis of chlorophyll. The protein Ycf54 was recently identified as part of the enzyme, and is associated with the plastid membrane associated subunit XanL. This work describes an analysis of the role of Ycf54 in the enzyme. It also demonstrates additional soluble factors remain unidentified.
ISSN:	1742-464X 1742-4658