Molecular cloning and characterization of 5-enolpyruvylshikimate-3-phosphate synthase gene from Convolvulus arvensis L

5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS), the target enzyme for glyphosate inhibition, catalyzes an essential step in the shikimate pathway for aromatic amino acid biosynthesis. The full-length cDNA of 1,751 nucleotides (CaEPSPS, Genbank accession number: EU698030) from Convolvulus arvens...

Full description

Saved in:
Bibliographic Details
Published in:Molecular biology reports 2014, Vol.41 (4), p.2077-2084
Main Authors: Huang, Zhao-Feng, Zhang, Chao-Xian, Huang, Hong-Juan, Wei, Shou-Hui, Liu, Yan, Cui, Hai-Lan, Chen, Jing-Chao, Yang, Long, Chen, Jin-Yi
Format: Article
Language:English
Subjects:
3-Phosphoshikimate 1-Carboxyvinyltransferase - genetics
3-Phosphoshikimate 1-Carboxyvinyltransferase - metabolism
Amino Acid Sequence
amino acids
Animal Anatomy
Animal Biochemistry
Arabidopsis
Base Sequence
Biomedical and Life Sciences
biosynthesis
Cloning
Cloning, Molecular
complementary DNA
Convolvulus - classification
Convolvulus - genetics
Convolvulus - metabolism
Convolvulus arvensis
DNA, Complementary - chemistry
DNA, Complementary - genetics
Drug Resistance - genetics
Enzyme Activation
Enzymes
Flowers & plants
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Plant
genes
Glycine - analogs & derivatives
Glycine - pharmacology
Glyphosate
Herbicides - pharmacology
Histology
isoelectric point
leaves
Life Sciences
Molecular biology
molecular cloning
Molecular Sequence Data
molecular weight
Morphology
nucleotides
Phenotype
Phylogeny
Plants, Genetically Modified
polypeptides
Promoter Regions, Genetic
proteins
roots
Sequence Alignment
shikimate pathway
stems
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS), the target enzyme for glyphosate inhibition, catalyzes an essential step in the shikimate pathway for aromatic amino acid biosynthesis. The full-length cDNA of 1,751 nucleotides (CaEPSPS, Genbank accession number: EU698030) from Convolvulus arvensis was cloned and characterized. The CaEPSPS encodes a polypeptide of 520 amino acids with a calculated molecular weight of 55.5 kDa and an isoelectric point of 7.05. The results of homology analysis revealed that CaEPSPS showed highly homologous with EPSPS proteins from other plant species. Tissue expression pattern analysis indicated that CaEPSPS was constitutively expressed in stems, leaves and roots, with lower expression in roots. CaEPSPS expression level could increase significantly with glyphosate treatment, and reached its maximum at 24 h after glyphosate application. We fused CaEPSPS to the CaMV 35S promoter and introduced the chimeric gene into Arabidopsis. The resultant expression of CaEPSPS in transgenic Arabidopsis plants exhibited enhanced tolerance to glyphosate in comparison with control.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-014-3056-2