Multi‐gene phylogenetic analysis of south‐east Asian pest members of the Bactrocera dorsalis species complex (Diptera: Tephritidae) does not support current taxonomy

Bactrocera dorsalis sensu stricto, B. papayae, B. philippinensis and B. carambolae are serious pest fruit fly species of the B. dorsalis complex that predominantly occur in south‐east Asia and the Pacific. Identifying molecular diagnostics has proven problematic for these four taxa, a situation that...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied entomology (1986) 2014-05, Vol.138 (4), p.235-253
Main Authors: Boykin, L. M, Schutze, M. K, Krosch, M. N, Chomič, A, Chapman, T. A, Englezou, A, Armstrong, K. F, Clarke, A. R, Hailstones, D, Cameron, S. L
Format: Article
Language:English
Subjects:
Bactrocera carambolae
Bactrocera dorsalis
Biological and medical sciences
biological speciation
biosecurity
data collection
Diptera
fruit flies
fruit fly
Fundamental and applied biological sciences. Psychology
internal transcribed spacers
loci
monophyly
multi-gene phylogeny
pest management
pests
Phytopathology. Animal pests. Plant and forest protection
probability
Protozoa. Invertebrates
quarantine
species delimitation
taxonomy
Tephritidae
trade
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bactrocera dorsalis sensu stricto, B. papayae, B. philippinensis and B. carambolae are serious pest fruit fly species of the B. dorsalis complex that predominantly occur in south‐east Asia and the Pacific. Identifying molecular diagnostics has proven problematic for these four taxa, a situation that cofounds biosecurity and quarantine efforts and which may be the result of at least some of these taxa representing the same biological species. We therefore conducted a phylogenetic study of these four species (and closely related outgroup taxa) based on the individuals collected from a wide geographic range; sequencing six loci (cox1, nad4‐3′, CAD, period, ITS1, ITS2) for approximately 20 individuals from each of 16 sample sites. Data were analysed within maximum likelihood and Bayesian phylogenetic frameworks for individual loci and concatenated data sets for which we applied multiple monophyly and species delimitation tests. Species monophyly was measured by clade support, posterior probability or bootstrap resampling for Bayesian and likelihood analyses respectively, Rosenberg's reciprocal monophyly measure, P(AB), Rodrigo's (P(RD)) and the genealogical sorting index, gsi. We specifically tested whether there was phylogenetic support for the four ‘ingroup’ pest species using a data set of multiple individuals sampled from a number of populations. Based on our combined data set, Bactrocera carambolae emerges as a distinct monophyletic clade, whereas B. dorsalis s.s., B. papayae and B. philippinensis are unresolved. These data add to the growing body of evidence that B. dorsalis s.s., B. papayae and B. philippinensis are the same biological species, which poses consequences for quarantine, trade and pest management.
ISSN:0931-2048
1439-0418
DOI:10.1111/jen.12047