Potential of Ni(II)-NTA-Modified Poly(ethylene imine) Glycopolymers as Carrier System for Future Dendritic Cell-Based Immunotherapy

Dendritic cells (DCs) play a crucial role in the development of cell-mediated immunotherapy due to their ability to induce and maintain strong immune responses. In our study, we evaluated a biocompatible Ni(II)-NTA-modified poly(ethylene imine) dendritic glycopolymer (Ni(II)-NTA-DG) as new carrier s...

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Bibliographic Details
Published in:Biomacromolecules 2014-03, Vol.15 (3), p.957-967
Main Authors: Hauptmann, N, Pion, M, Wehner, R, Muñoz-Fernández, M.-Á, Schmitz, M, Voit, B, Appelhans, D
Format: Article
Language:English
Subjects:
Applied sciences
Biological and medical sciences
Cell Differentiation - genetics
Cell Differentiation - immunology
Dendritic Cells - chemistry
Dendritic Cells - cytology
Dendritic Cells - immunology
Exact sciences and technology
Flow Cytometry
General pharmacology
Humans
Imines - chemistry
Imines - immunology
Immunotherapy
Interleukin-6 - chemistry
Interleukin-6 - metabolism
Interleukin-8 - chemistry
Interleukin-8 - metabolism
Lipopolysaccharides - metabolism
Medical sciences
Monocytes - cytology
Organic polymers
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Physicochemistry of polymers
Polyethylenes - chemistry
Properties and characterization
Special properties (catalyst, reagent or carrier)
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Summary:Dendritic cells (DCs) play a crucial role in the development of cell-mediated immunotherapy due to their ability to induce and maintain strong immune responses. In our study, we evaluated a biocompatible Ni(II)-NTA-modified poly(ethylene imine) dendritic glycopolymer (Ni(II)-NTA-DG) as new carrier system to increase the antigen uptake into iDCs for future DC-based immunotherapy. Ni(II)-NTA-DG led to an increase in His6-Gp160 uptake in monocytes and iDCs, where His6-Gp160 is localized in the early endosomal and lysosomal compartments. Ni(II)-NTA-DG and the formed polyplexes induced an activation of iDCs, showing an increasing expression of costimulatory molecules CD86, CD80, and proinflammatory cytokines IL-6 and IL-8. Beside no influencing effect of Ni(II)-NTA-DG and polyplexes on the maturation of antigen-bearing DCs, the mature peptide bearing DCs remained their ability to migrate along a gradient of CCR7 ligands. Thus, Ni(II)-NTA-DG with advancing biological properties is a promising carrier system for the future application in DC-based immunotherapy.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm401845b