An enzyme containing microemulsion based on skin friendly oil and surfactant as decontamination medium for organo phosphates: Phase behavior, structure, and enzyme activity

[Display omitted] •A skin friendly microemulsion could be made with technical grade components.•The obtained microemuslion follows the behavior known from model systems.•The enzyme DFPase still is active in this microemulsion despite the alcohol content.•The activity still is sufficiently high to be...

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Bibliographic Details
Published in:Journal of colloid and interface science 2014-01, Vol.413 (413), p.127-132
Main Authors: Stehle, Ralf, Schulreich, Christoph, Wellert, Stefan, Gäb, Jürgen, Blum, Marc-Michael, Kehe, Kai, Richardt, Andras, Lapp, Alain, Hellweg, Thomas
Format: Article
Language:English
Subjects:
Chemistry
Colloidal state and disperse state
Cosmetics
Decontamination
DFPase
Dynamic light scattering
Dynamical systems
Dynamics
Emulsions
Emulsions. Microemulsions. Foams
Enzymes
Exact sciences and technology
General and physical chemistry
Microemulsion
Microemulsions
Molecular Structure
Oils - chemistry
Organophosphorus Compounds - chemistry
Protein
Sarin
Skin
Small angle neutron scattering
Surface-Active Agents - chemistry
Surfactants
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Summary:[Display omitted] •A skin friendly microemulsion could be made with technical grade components.•The obtained microemuslion follows the behavior known from model systems.•The enzyme DFPase still is active in this microemulsion despite the alcohol content.•The activity still is sufficiently high to be useful for skin decontamination. The present contribution presents a microemulsion system containing cosmetic oil and sugar surfactant and the enzyme diisopropyl fluorophosphatase (DFPase) as active agent for the decontamination of human skin. The bicontinuous structure and the physical properties of the microemulsion are characterized by dynamic light scattering and small angle neutron scattering. The DFPase from the squid Loligo vulgaris is catalyzing the hydrolysis of highly toxic organophosphates. The effect of the enzyme on the structure of the microemulsion is investigated. Moreover, the enzyme/microemulsion system is also studied with respect to its activity using nuclear magnetic resonance spectroscopy leading to promising results. A fast decomposition of the nerve agent sarin is achieved.
ISSN:0021-9797
1095-7103
DOI:10.1016/j.jcis.2013.09.027