Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920

Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty, the present study adopts response surface methodology to optimize the chemical compositions of protective agents to seek for maximum viability of Bifidobacterium longum BIOMA 5920 during freeze-dr...

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Bibliographic Details
Published in:Chinese journal of chemical engineering 2012-10, Vol.20 (5), p.930-936
Main Author: 杨婵媛 朱晓丽 范代娣 米钰 骆艳娥 惠俊峰 苏然
Format: Article
Language:English
Subjects:
Bifidobacterium
Bifidobacterium longum
Box-Behnken设计
freeze-drying
protective agent
response surface methodology
viability
保护剂
农业部
冷冻干燥过程
化学成分
变量优化
科学研究所
长双歧杆菌
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Summary:Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty, the present study adopts response surface methodology to optimize the chemical compositions of protective agents to seek for maximum viability of Bifidobacterium longum BIOMA 5920 during freeze-drying. Through the compara- tive analysis of single protectant, the complex protective agents show better effect on the Bifidobacterium viability. Human-like collagen （HLC）, trehalose and glycerol are confirmed as significant factors by Box-Behnken Design. The optimized formula for these three variables is tested as follows： HLC 1.23%, trehalose 11.50% and glycerol 4.65%. Under this formula, the viability is 88.23%, 39.67% higher in comparison to the control. The viable count is 1.07×10 9 cfu·g-1 , greatly exceeding the minimum viable count requirement （10 6 cfu·g-1 ）.
ISSN:1004-9541
2210-321X
DOI:10.1016/S1004-9541(12)60420-0