TAT conjugated cationic noble metal nanoparticles for gene delivery to epidermal stem cells

TAT conjugated cationic noble metal nanoparticles for gene delivery to epidermal stem cells

Abstract Most nonviral gene delivery systems are not efficient enough to manipulate the difficult-to-transfect cell types, including non-dividing, primary, neuronal or stem cells, due to a lack of an intrinsic capacity to enter the membrane and nucleus, release its DNA payload, and activate transcri...

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Bibliographic Details
Published in:Biomaterials 2014-07, Vol.35 (21), p.5605-5618
Main Authors: Peng, Li-Hua, Niu, Jie, Zhang, Chen-Zhen, Yu, Wei, Wu, Jia-He, Shan, Ying-Hui, Wang, Xia-Rong, Shen, You-Qing, Mao, Zheng-Wei, Liang, Wen-Quan, Gao, Jian-Qing
Format: Article
Language:eng
Subjects:
Advanced Basic Science
Animals
Cations - chemistry
Cell-Penetrating Peptides - chemistry
Cells, Cultured
Dentistry
DNA - chemistry
Epidermis - cytology
Gene delivery
Gene Transfer Techniques
Genetic Therapy
Gold - chemistry
Metal nanoparticles
Metal Nanoparticles - chemistry
Particle Size
Peptide Fragments - chemistry
Rats
Silver - chemistry
Stem cell
Stem Cells
TAT conjugation
Transfection
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Summary:Abstract Most nonviral gene delivery systems are not efficient enough to manipulate the difficult-to-transfect cell types, including non-dividing, primary, neuronal or stem cells, due to a lack of an intrinsic capacity to enter the membrane and nucleus, release its DNA payload, and activate transcription. Noble metal nanoclusters have emerged as a fascinating area of widespread interest in nanomaterials. Herein, we report the synthesis of the TAT peptide conjugated cationic noble metal nanoparticles (metal NPs@PEI-TAT) as highly efficient carriers for gene delivery to stem cells. The metal NPs@PEI-TAT integrate the advantages of metal NPs and peptides: the presence of metal NPs can effectively decrease the cytotoxicity of cationic molecules, making it possible to apply them in biological systems, while the cell penetrating peptides are essential for enhanced cellular and nucleus entry to achieve high transfection efficiency. Our studies provide strong evidence that the metal NPs@PEI-TAT can be engineered as gene delivery agents for stem cells and subsequently enhance their directed differentiation for biomedical application.
ISSN:0142-9612
1878-5905