Plasma pharmacokinetics and lung distribution of tetrahydropalmatine after topical application of cold asthma recipe extract: Feishu (BL 13) versus Non-Feishu acupoint

Acupoint application of cold asthma recipe (CAR) was a traditional Chinese medicine (TCM) method, widely used as an alternative medicine for clinical prevention of the common winter diseases of asthma and bronchitis. Tetrahydropalmatine (THP) was a main active ingredient of CAR extract. The aim of t...

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Published in:Journal of ethnopharmacology 2014-04, Vol.153 (2), p.454-460
Main Authors: Lin, Yuan-yuan, Wang, Yin-ping, Lu, Hao-yang, Guo, Xiu-cai, Liu, Xia, Wu, Chuan-bin, Xu, Yue-hong
Format: Article
Language:English
Subjects:
Acupoint
Acupuncture Points
Administration, Topical
Animals
Asthma - blood
Asthma - drug therapy
Berberine Alkaloids - blood
Berberine Alkaloids - pharmacokinetics
Drugs, Chinese Herbal - administration & dosage
Drugs, Chinese Herbal - pharmacokinetics
Female
Lung - drug effects
Lung - metabolism
Lung distribution
Medicine, Chinese Traditional - methods
Mice
Pharmacokinetics
Random Allocation
Rats
Rats, Wistar
Tetrahydropalmatine
UPLC-MS/MS
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Summary:Acupoint application of cold asthma recipe (CAR) was a traditional Chinese medicine (TCM) method, widely used as an alternative medicine for clinical prevention of the common winter diseases of asthma and bronchitis. Tetrahydropalmatine (THP) was a main active ingredient of CAR extract. The aim of this study is to compare plasma pharmacokinetics and lung distribution of THP between Feishu (FS) acupoint (BL 13) and Non-Feishu (NFS) acupoint application of CAR extract by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The extract of CAR was topically administrated in FS and NFS acupoint of rats for plasma pharmacokinetics, and topically administrated in FS and NFS acupoint of mice for lung distribution. The plasma and lung homogenates were pretreated by protein precipitation with acetonitrile. Chromatographic separation was performed on an ACQUITY UPLC BEH C18 column with a mobile phase consisted of 0.1% formic acid in acetonitrile and 0.1% formic acid in water. The detection was accomplished by multiple-reaction monitoring (MRM) scanning in the positive electrospray ionization (ESI+) mode. All pharmacokinetic parameters were estimated by non-compartmental analysis. A sensitive, accurate and precise UPLC-MS/MS method was successfully established for determination of THP in 100μL plasma and lung homogenate. The lower limit of quantification (LLOQ) of THP was 0.05ng/mL and 0.072ng/mL, respectively. The pharmacokinetic results manifested that THP was absorbed and eliminated slowly in plasma. Additionally, it was found that there was significantly higher amount of THP absorbed into blood and lung after FS acupoint application compared to NFS acupoint application. Both of the rat plasma pharmacokinetics and mice lung distribution of THP could support that FS acupoint application of CAR extract has greater advantages of absorption into the blood circulation and distribution in target tissue over NFS acupoint application. The results might be helpful in providing a rational explanation for why the TCM chose the acupoint application and elucidating the underlying mechanism of this treatment. [Display omitted]
ISSN:0378-8741
1872-7573
DOI:10.1016/j.jep.2014.03.009