Adhesion of B. subtilis spores and vegetative cells onto stainless steel – DLVO theories and AFM spectroscopy

[Display omitted] •Interactions between bacteria and stainless steel surfaces were quantified.•Classical DLVO theory and extended DLVO approach were used together with Atomic Force Microscopy.•Spores showed stronger attraction interactions to stainless steel compared to their vegetative cells.•DLVO...

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Bibliographic Details
Published in:Journal of colloid and interface science 2013-09, Vol.405, p.233-241
Main Authors: Harimawan, Ardiyan, Zhong, Shaoping, Lim, Chwee-Teck, Ting, Yen-Peng
Format: Article
Language:English
Subjects:
Adhesion
Adhesion force
Adhesion tests
AFM
Atomic force microscopy
Attachment
Austenitic stainless steels
B. subtilis
Bacillus subtilis
Bacillus subtilis - physiology
bacteria
Bacterial Adhesion
biofilm
Biofilms
Chemistry
DLVO theories
energy
environmental factors
Exact sciences and technology
General and physical chemistry
Hydrodynamics
Hydrophobic and Hydrophilic Interactions
Hydrophobicity
Microscopy, Atomic Force
Microscopy, Electron, Scanning
Models, Theoretical
Spectroscopy
Spore
Spores
Spores, Bacterial - physiology
Stainless Steel
Stainless steels
Surface physical chemistry
Surface Properties
vegetative cells
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Summary:[Display omitted] •Interactions between bacteria and stainless steel surfaces were quantified.•Classical DLVO theory and extended DLVO approach were used together with Atomic Force Microscopy.•Spores showed stronger attraction interactions to stainless steel compared to their vegetative cells.•DLVO and xDLVO theories predict vegetative cells show less permanent attachment on surfaces compared to spores. Interactions between the bacterium Bacillus subtilis (either as vegetative cells or as spores) and stainless steel 316 (SS-316) surfaces were quantified using the classical Derjaguin–Landau–Verwey–Overbeek (DLVO) theory and extended DLVO (xDLVO) approach in conjunction with live force spectroscopy using an Atomic Force Microscope (AFM). The xDLVO approach accounts for acid–base (polar) interactions that are not considered in the classical DLVO theory. AFM results revealed that spores manifested stronger attraction interactions to stainless steel compared to their vegetative cells counterparts due to lower energy barrier as predicted by both the theoretical approaches as well as the higher hydrophobicity on the spore surfaces. Both DLVO and xDLVO theories predict that vegetative cells manifest weaker attachment on the surfaces compared to spores. Results of AFM force measurement corroborate these findings; spores recorded significantly higher adhesion force (2.92±0.4nN) compared to vegetative cells (0.65±0.2nN). The adhesion of spores presents greater challenges in biofilm control owing to its stronger attachment and persistence when the spores are formed under adverse environmental conditions.
ISSN:0021-9797
1095-7103
DOI:10.1016/j.jcis.2013.05.031