Design and implementation of an imprinted material for the extraction of the endocrine disruptor bisphenol A from milk

•An imprinted polymer for bisphenol A was designed with hydroquinone as template.•MIP-SPE method for bisphenol A in milk was developed and validated.•Testing milk samples, bisphenol A was identified in one at a level of 176μgkg−1.•BPA self-association effects were examined with crystallography.•Pre-...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2013-07, Vol.931, p.164-169
Main Authors: O’Mahony, John, Moloney, Mary, McCormack, Martin, Nicholls, Ian A., Mizaikoff, Boris, Danaher, Martin
Format: Article
Language:English
Subjects:
Animals
Benzhydryl Compounds - analysis
Benzhydryl Compounds - chemistry
Benzhydryl Compounds - isolation & purification
Bisphenol A
Cattle
Chromatography, High Pressure Liquid - methods
Cleaning
Design engineering
detection limit
Endocrine Disruptors - analysis
Endocrine Disruptors - chemistry
Endocrine Disruptors - isolation & purification
endocrine-disrupting chemicals
Extraction
fluorescence
hemorrhage
High performance liquid chromatography
HPLC-FLD
Hydroquinones - chemistry
Imprinted polymers
Limit of Detection
Milk
Milk - chemistry
MIP
Molecular dynamics
Molecular Imprinting
Phenols - analysis
Phenols - chemistry
Phenols - isolation & purification
polymers
Polymers - chemistry
Reproducibility of Results
Solid Phase Extraction
SPE
surveys
Tandem Mass Spectrometry
UHPLC–MS/MS
X-ray diffraction
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Summary:•An imprinted polymer for bisphenol A was designed with hydroquinone as template.•MIP-SPE method for bisphenol A in milk was developed and validated.•Testing milk samples, bisphenol A was identified in one at a level of 176μgkg−1.•BPA self-association effects were examined with crystallography.•Pre-polymerization interactions were studied using molecular dynamics simulations. This paper describes the determination of bisphenol A (BPA) in milk samples, using a novel molecularly imprinted polymer. The imprinted polymer was developed using a rational design approach, and pre-polymerization interactions were investigated using molecular dynamics simulations and X-ray crystallography. A hydroquinone-imprinted polymer was used for solid phase extraction (SPE) clean-up of samples. BPA was quantified by high performance liquid chromatography (HPLC) and fluorescence (FLD) detection. Following validation, the method described was capable of determining bisphenol A in milk down to a limit of detection of 1.32μgkg−1. The method was applied to a survey (n=27) of commercial milk products; BPA was detected in one of the samples, at a level of 176μgkg−1. Test results were confirmed by a parallel UHPLC–MS/MS analytical method. This demonstrates the utility of the hydroquinone-imprinted polymer for application to selective sample clean-up and analysis of bisphenol A in milk, avoiding possible detrimental affects associated with template bleeding and without the need for expensive or difficult-to-obtain template.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2013.05.025