Interaction between Rice stripe virus Disease- Specific Protein and Host PsbP Enhances Virus Symptoms

Rice stripe virus （RSV） causes severe diseases in Oryza sativa （rice） in many Eastern Asian countries. Diseasespecific protein （SP） of RSV is a non-structural protein and its accumulation level in rice plant was shown to determine the severity of RSV symptoms. Here, we present evidence that expressi...

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Bibliographic Details
Published in:	Molecular plant 2014-04, Vol.7 (4), p.691-708
Main Authors:	Kong, Lingfang, Wu, Jianxiang, Lu, Lina, Xu, Yi, Zhou, Xueping
Format:	Article
Language:	eng
Subjects:	Chloroplasts Disease disease-specific protein interaction Nicotiana - metabolism Nicotiana - virology Oryza - genetics Oryza - metabolism Oryza - virology Plant Diseases - genetics Plant Diseases - virology Plant Proteins - genetics Plant Proteins - metabolism Potexvirus - pathogenicity Proteins PsbP Rice stripe virus symptom 主机叶绿体结构呼吸道合胞病毒水稻条纹病毒特异性蛋白病毒病害症状马铃薯X病毒
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Summary:	Rice stripe virus （RSV） causes severe diseases in Oryza sativa （rice） in many Eastern Asian countries. Diseasespecific protein （SP） of RSV is a non-structural protein and its accumulation level in rice plant was shown to determine the severity of RSV symptoms. Here, we present evidence that expression of RSV SP alone in rice or Nicotiana benthamiana did not produce visible symptoms. Expression of SP in these two plants, however, enhanced RSV- or Potato virus X （PVX）- induced symptoms. Through yeast two-hybrid screening, GST pull-down, and bimolecular fluorescence complementation assays, we demonstrated that RSV SP interacted with PsbP, a 23-kDa oxygen-evolving complex protein, in both rice and N. benthamiana. Furthermore, our investigation showed that silencing of PsbP expression in both plants increased disease symptom severity and virus accumulation. Confocal microscopy using N, benthamiana protoplast showed that PsbP accu- mulated predominantly in chloroplast in wild-type N. benthamiana cells. In the presence of RSV SP, most PsbP was recruited into cytoplasm of the assayed cells. In addition, accumulation of SP during RSV infection resulted in alterations of chloroplast structure and function. Our findings shed light on the molecular mechanism underlying RSV disease symptom development.
ISSN:	1674-2052 1752-9867