Biosensor based on nanocomposite material for pathogenic virus detection

•DNA biosensor based on DNA/CHIT/CNTs nanocomposite for pathogen virus detection was developed in this work.•DNA sensor can detect a target DNA concentration as low as 0.01×10−12M.•The sensitivity of the prepared sensor was 52.57kΩ/fM. This paper introduces a DNA biosensor based on a DNA/chitosan/mu...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2014-03, Vol.115, p.176-181
Main Authors: Van Thu, Vu, Dung, Phuong Trung, Tam, Le Thi, Tam, Phuong Dinh
Format: Article
Language:English
Subjects:
Base Sequence
Biosensing Techniques - methods
Biosensor
Carbon nanotube
Computer Simulation
Dielectric Spectroscopy
DNA
DNA - analysis
DNA sensor
Electrochemical Techniques
Electrodes
Functionalization
Nanocomposites - chemistry
Nanotubes, Carbon - chemistry
Nucleic Acid Hybridization
Recycling
Viruses - isolation & purification
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Summary:•DNA biosensor based on DNA/CHIT/CNTs nanocomposite for pathogen virus detection was developed in this work.•DNA sensor can detect a target DNA concentration as low as 0.01×10−12M.•The sensitivity of the prepared sensor was 52.57kΩ/fM. This paper introduces a DNA biosensor based on a DNA/chitosan/multi-walled carbon nanotube nanocomposite for pathogenic virus detection. An easy, cost-effective approach to the immobilization of probe DNA sequences on the sensor surface was performed. Cyclic voltammograms were used to characterize the probe DNA sequence immobilization. Complementary sequence hybridization was examined by electrochemical impedance spectroscopy. Results revealed that the developed DNA sensor can detect a target DNA concentration as low as 0.01×10−12M. The sensitivity of the prepared sensor was 52.57kΩ/fM. The reusability and storage stability of the DNA sensor were also investigated. Results showed that the electron-transfer resistance decreased to approximately 35% after 8 weeks and to approximately 80% after 12 weeks of storage.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2013.11.016