PHENOTYPIC DETECTION OF METALLO-β-LACTAMASE PRODUCING ENTEROBACTERIACEAE

Phenotypic Detection of Metallo- beta -lactamase-producing Enterobacteriaceae from patients of a Tertiary Care Hospital, Ahmedabad. The study was conducted over period of one year, from July 2011 to June 2012. A total of 1,072 Enterobacteriaceae isolates from various clinical samples of indoor patie...

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Bibliographic Details
Published in:International journal of microbiology research 2012-12, Vol.4 (9), p.326-329
Main Authors: P, JAIN, V, GANDHI, K, PATEL, G, MODI, R, PARMAR, S, SONI, MM, VEGAD
Format: Article
Language:English
Subjects:
Enterobacteriaceae
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Summary:Phenotypic Detection of Metallo- beta -lactamase-producing Enterobacteriaceae from patients of a Tertiary Care Hospital, Ahmedabad. The study was conducted over period of one year, from July 2011 to June 2012. A total of 1,072 Enterobacteriaceae isolates from various clinical samples of indoor patients were included in the study. All isolates were non-duplicate. Antimicrobial susceptibility of all the isolates was performed by the disc diffusion method. Metallo beta lactamase (MBL) production was detected in imipenem-resistant isolates by phenotypic tests. The Imipenem (IMP)-EDTA combined disc diffusion test was used. MBL producing Enterobacteriaceae isolates were 2.35%. Most common MBL producing organism was Klebsiel-la pneumoniae, from swab and urine of patients collected from intensive care unit (ICU). In present study, the imipenem-resistant isolates also show resistance to other groups of antibiotics, which is a uniquely seen with MBLs producers that show a broad-spectrum re-sistance profile. The majority of these MBL isolates were from patients of the ICU and post-operative wards; areas where the majority of critically ill patients are concentrated.
ISSN:0975-5276
0975-9174
DOI:10.9735/0975-5276.4.9.326-329