Assessing experimental visceral pain in dairy cattle: A pilot, prospective, blinded, randomized, and controlled study focusing on spinal pain proteomics

Few studies have verified the validity of behavioral and physiological methods of pain assessment in cattle. This prospective, blinded, randomized controlled experimental study aimed to validate different methods of pain assessment during acute and chronic (up to 21 d postintervention) conditions in...

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Bibliographic Details
Published in:Journal of dairy science 2014-04, Vol.97 (4), p.2118-2134
Main Authors: Rialland, P., Otis, C., de Courval, M.-L., Mulon, P.-Y., Harvey, D., Bichot, S., Gauvin, D., Livingston, A., Beaudry, F., Hélie, P., Frank, D., del Castillo, J.R.E., Troncy, E.
Format: Article
Language:English
Subjects:
Analgesia - methods
Analgesia - veterinary
Analgesics - therapeutic use
animal behavior
Animals
Biomarkers - cerebrospinal fluid
Catecholamines - cerebrospinal fluid
Cattle
central sensitization
Pain Management - veterinary
Pain Measurement - methods
Pain Measurement - veterinary
pain metrology
Pilot Projects
Prealbumin - cerebrospinal fluid
preemptive multimodal analgesia
Prospective Studies
Proteomics
Visceral Pain - diagnosis
Visceral Pain - drug therapy
Visceral Pain - veterinary
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Summary:Few studies have verified the validity of behavioral and physiological methods of pain assessment in cattle. This prospective, blinded, randomized controlled experimental study aimed to validate different methods of pain assessment during acute and chronic (up to 21 d postintervention) conditions in dairy cattle, in response to 3 analgesic treatments for traumatic reticuloperitonitis. Cerebrospinal fluid (CSF) biomarkers and mechanical sensitization were measured as indicators of centralized pain. Proteomics in the CSF were examined to detect specific (to pain intensity) and sensitive (responsive to analgesia) markers. Recordings of spontaneous behavior with video analysis, telemetered motor activity, pain scales, electrodermal activity, and plasma cortisol concentration were quantified at regular intervals. Cows were assigned to group 1 (n=4, standard control receiving aspirin), group 2 (n=5, test group receiving preemptive tolfenamic acid), or group 3 (n=3, positive control receiving preemptive multimodal analgesia composed of epidural morphine, plus tolfenamic acid and butorphanol). Rescue analgesia was administered as needed. Generalized estimating equations tested group differences and the influence of rescue analgesia on the measurements. All 3 groups demonstrated a long-term decrease in a CSF protein identified as transthyretin. The decrease in transthyretin expression inversely correlated with the expected level of analgesia (group 1
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.2013-7142