In vitro and in vivo antioxidant activity of a fructan from the roots of Arctium lappa L

To explore new antioxidant resource from food, a water-soluble polysaccharide (ALP1) was extracted and purified from the roots of Arctium lappa L. (A. lappa L.) through hot water extraction followed by ethanol precipitation, ion-exchange chromatography and gel filtration. The antioxidant activity of...

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Bibliographic Details
Published in:International journal of biological macromolecules 2014-04, Vol.65, p.446-453
Main Authors: Liu, Wei, Wang, Jiajia, Zhang, Zhenzhen, Xu, Jinnan, Xie, Zhuohong, Slavin, Margaret, Gao, Xiangdong
Format: Article
Language:English
Subjects:
Animals
Antioxidant activity
Arctium - chemistry
Arctium lappa L
Chemical Phenomena
Female
Free Radical Scavengers - chemistry
Free Radical Scavengers - pharmacology
Free Radicals - chemistry
Fructan
Fructans - chemistry
Fructans - pharmacology
Mice
Plant Roots - chemistry
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Summary:To explore new antioxidant resource from food, a water-soluble polysaccharide (ALP1) was extracted and purified from the roots of Arctium lappa L. (A. lappa L.) through hot water extraction followed by ethanol precipitation, ion-exchange chromatography and gel filtration. The antioxidant activity of ALP1 was then evaluated in vitro and in vivo. ALP1 was characterized as a fructan composed of fructose and glucose in the ratio of 13.0:1.0, with an average molecular weight of 4600Da. The linkages in ALP1 were →1)-Fruf-(2→, Fruf-(2→ and Glcp-(1→. In vitro antioxidant assays demonstrated that ALP1 possessed moderate ABTS+ scavenging activity, strong hydroxyl radical scavenging activity and strong ferrous ion chelating activity. In in vivo antioxidant assays, ALP1 administration significantly enhanced antioxidant enzyme activities and total antioxidant capacity, as well as decreased the levels of malondialdehyde (MDA) in both the serum and liver of aging mice. These results suggest that ALP1 has potential as a novel natural antioxidant in food industry and pharmaceuticals.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2014.01.062