Highly efficient colorimetric detection of target cancer cells utilizing superior catalytic activity of graphene oxide-magnetic-platinum nanohybrids

Enzyme-linked immunosorbent assays (ELISAs) have most widely been applied in immunoassays for several decades. However, several unavoidable limitations (e.g., instability caused by structural unfolding) of natural enzymes have hindered their widespread applications. Here, we describe a new nanohybri...

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Bibliographic Details
Published in:Nanoscale 2014-01, Vol.6 (3), p.1529-1536
Main Authors: Kim, Moon Il, Kim, Min Su, Woo, Min-Ah, Ye, Youngjin, Kang, Kyoung Suk, Lee, Jinwoo, Park, Hyun Gyu
Format: Article
Language:English
Subjects:
Assaying
Benzidines - chemistry
Breast Neoplasms - diagnosis
Breast Neoplasms - metabolism
Catalysis
Colorimetry - methods
Female
Graphite - chemistry
Humans
Immunoassay
Kinetics
Magnetics
Metal Nanoparticles - chemistry
Microscopy, Electron, Scanning Transmission
Nanotechnology
Oxides - chemistry
Peroxidase - chemistry
Peroxidases - chemistry
Platinum - chemistry
Sensitivity and Specificity
X-Ray Diffraction
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Summary:Enzyme-linked immunosorbent assays (ELISAs) have most widely been applied in immunoassays for several decades. However, several unavoidable limitations (e.g., instability caused by structural unfolding) of natural enzymes have hindered their widespread applications. Here, we describe a new nanohybrid consisting of Fe₃O₄ magnetic nanoparticles (MNPs) and platinum nanoparticles (Pt NPs), simultaneously immobilized on the surface of graphene oxide (GO). By synergistically integrating highly catalytically active Pt NPs and MNPs on GO whose frameworks possess high substrate affinity, the nanohybrid is able to achieve up to a 30-fold higher maximal reaction velocity (V(max)) compared to that of free GO for the colorimetric reaction of the peroxidase substrate, 3,3',5,5'-tetramethylbenzidine (TMB), and enable rapid detection of target cancer cells. Specifically, using this new assay system, clinically important breast cancer cells are detected in a 5 min time period at room temperature with high specificity and sensitivity. The remarkably high capability to catalyze oxidation reactions could allow the nanohybrid to replace conventional peroxidase-based immunoassay systems as part of new, rapid, robust and convenient assay systems which can be widely utilized for the identification of important target molecules.
ISSN:2040-3364
2040-3372
DOI:10.1039/c3nr05539f